Mitogen-Activated Protein Kinase 9 (MAPK9) (phospho) antibody

Antigen: Mitogen-Activated Protein Kinase 9 (MAPK9)

Synonyms: JNK2, SAPK, p54a, JNK2A, JNK2B, PRKM9, JNK-55, JNK2BETA, p54aSAPK, JNK2ALPHA, MAPK9, MGC137545, MAP kinase 9, MPK9

Epitope: phospho

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Catalog no.: ABIN223283

Additional Information

Alternative name: Phospho-JNK/SAPK

Gene ID: 5601

Format: Purified

Description: 100 ug rabbit polyclonal antibody. Reacts with human/mouse/rat. Detects p46 and p54 JNK/SAPK dually phosphorylated at Thr183 and Tyr185. Tested in WB/IP/IHC. Synonyms: Phospho-JNK, SAPK, Anti-Phospho-JNK, Anti-SAPK, Phospho-JNK antibody, Anti-Phospho-SAPK, Phospho-JNK, JNK, MEKK1, SEK1, MKK4, SAPK, c-Jun, ATF-2 Synonyms: Phospho-JNK, SAPK, Anti-Phospho-JNK, Anti-SAPK, Phospho-JNK antibody, Anti-Phospho-SAPK, Phospho-JNK, JNK, MEKK1, SEK1, MKK4, SAPK, c-Jun, ATF-2

Specificity: Detects p46 and p54 JNK/SAPK dually phosphorylated at Thr183 and Tyr185

Application Details

Application Notes: The antibody can be used for Western blotting (1-2 ug/ml), immunoprecipitation (10-20 ug/ml), immunocytochemistry (10-20 ug/ml), and immunohistochemistry (20-30 ug/ml). However, the optimal conditions should be determined individually.

Buffer: 100 ug (0.5 mg/ml) affinity purified rabbit anti-phospho-JNK/SAPK polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.

Storage: Store at -20 o C. For long-term storage, aliquot and freeze at -70 o C. Avoid repeated freeze/defrost cycles Shelf Life: 12 Months

Restrictions: For Research Use only

Publications

Publications: Ji, Chien, Lin et al.: "5-ALA mediated photodynamic therapy induces autophagic cell death via AMP-activated protein kinase." in: Molecular cancer, Vol. 9, pp. 91, 2010 (PubMed).