Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) antibody

Antigen: Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2)

Synonyms: TRKB, GP145-TrkB, Tkrb, trkB, AI848316, C030027L06Rik, TRKB1, RATTRKB1, trkb, xtrkb, trkb-b, MGC52547, NTRK2, Trk-B, MGC140769, ntrk2b, ntrk2, MGC99292, MGC145900, gp145-trkb

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN223287

Additional Information

Alternative name: TrkB

Gene ID: 25054

Immunogen: Synthetic peptide surrounding amino acid 810 of human TrkB

Format: Liquid

Isotype: IgG

Description: Due to the various splice variants, the trk proteins exist as variably glycosylated entities with the major forms having molecular weights of 140-145 kDa, 110 kDa and the unglycosylated form of 80 kDa. trkB contains 33.3% carbohydrate by weight representing modification on 10 of 12 N-glycosylation sites. The primary ligand for trkB is BDNF which induces the phosphorylation of the protein and subsequent binding of PLC-gamma via SH2 domains. TrkB may function to modulate neuronal responses to the neurotrophins acting through trkB such as BDNF.

Specificity: Detects TrkB in samples from human/rat origins

Application Details

Application Notes: The antibody can be used in Western blotting (0.5-4 ug/ml). Based on researchers feedback, it also works well in immunofluorescence (5-10 ug/ml). However, the optimal concentrations should be determined individually.

Handling Advice: The antibody solution should be gently mixed before use.

Concentration: 0.2 mg/mL

Purification: Affinity purified

Buffer: phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal

Preservative: Thimerosal

Storage: -20 °C

Expiry Date: 12 months

Restrictions: For Research Use only

Images

Western Blot Analysis of trkB expression in HL-60 Cell Lysate.

Publications

Jang, Liu, Chan et al.: "Deoxygedunin, a natural product with potent neurotrophic activity in mice." in: PLoS ONE, Vol. 5, Issue 7, pp. e11528, 2010 (PubMed).

Product: Jang, Liu, Pradoldej et al.: "N-acetylserotonin activates TrkB receptor in a circadian rhythm." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 8, pp. 3876-81, 2010 (PubMed).

Jang, Liu, Yepes et al.: "A selective TrkB agonist with potent neurotrophic activities by 7,8-dihydroxyflavone." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 6, pp. 2687-92, 2010 (PubMed).

Shen, Ghai, Sompol et al.: "N-acetyl serotonin derivatives as potent neuroprotectants for retinas." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 9, pp. 3540-5, 2012 (PubMed).