Actin, beta (ACTB) antibody

Antigen: Actin, beta (ACTB)

Synonyms: PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1

Clonality: Monoclonal (B11V08)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Pig (Porcine), Sheep (Ovine), Chicken, Rabbit, Cow (Bovine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN223292

Additional Information

Alternative name: Beta-Actin

Gene ID: 60

Immunogen: Synthetic peptide corresponding to the N-terminus of human beta-Actin.

Format: Purified

Isotype: IgG2a

Clone: B11V08

Description: Actin is expressed in all eukaryotic cells and is the major component of the cytoskeleton. At least six types of actin are present in mammalian tissues and fall into three classes. Alpha actin expression is limited to various types of muscle and it regulates contactile potentials for the muscle cells, whereas beta and gamma actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility.

Characteristics: 100 ug mouse anti-beta-Actin monoclonal antibody. Reacts with human/mouse/rat/pig/sheep/chicken/rabbit/bovine. Tested in WB/IF/IHC.

Specificity: The antibody recognizes 44 kDa beta-Actin in samples from human, mouse, rat, pig, sheep, chicken, rabbit, bovine origins.

Synonyms: PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1

Application Details

Application Notes: The antibody can be used for Western blotting (0.5-2 ug/ml), immunofluorescence (5-10 ug/ml) and immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually.

Buffer: 100 ug (0. 5 mg/ml) Protein G affinity purified mouse anti-beta-Actin monoclonal antibody in phosphate-buffered saline (PBS) containing 0.02% thimerosal.

Storage: Store at -20 o C. For long term storage, aliquot and freeze at

Restrictions: For Research Use only

Publications

Ellis, Gattoni-Celli, Mancia et al.: "The vitamin D3 transcriptomic response in skin cells derived from the Atlantic bottlenose dolphin." in: Developmental and comparative immunology, Vol. 33, Issue 8, pp. 901-12, 2009 (PubMed).

Liu, Song, Wang et al.: "Effect of chronic intermittent hypoxia on biological behavior and hypoxic-associated gene expression in lung cancer cells." in: Journal of cellular biochemistry, 2010 (PubMed).

Product: Fullerton, Hakimuddin, Bakovic: "Developmental and metabolic effects of disruption of the mouse CTP:phosphoethanolamine cytidylyltransferase gene (Pcyt2)." in: Molecular and cellular biology, Vol. 27, Issue 9, pp. 3327-36, 2007 (PubMed).

Weng, Chau, Hsieh et al.: "Lucidenic acid inhibits PMA-induced invasion of human hepatoma cells through inactivating MAPK/ERK signal transduction pathway and reducing binding activities of NF-kappaB and AP-1." in: Carcinogenesis, Vol. 29, Issue 1, pp. 147-56, 2008 (PubMed).

Wang, Allen, May et al.: "Enterococcus faecalis induces aneuploidy and tetraploidy in colonic epithelial cells through a bystander effect." in: Cancer research, Vol. 68, Issue 23, pp. 9909-17, 2008 (PubMed).

Harada, Itasaka, Kizaka-Kondoh et al.: "The Akt/mTOR pathway assures the synthesis of HIF-1alpha protein in a glucose- and reoxygenation-dependent manner in irradiated tumors." in: The Journal of biological chemistry, Vol. 284, Issue 8, pp. 5332-42, 2009 (PubMed).

Carlsen, Donohue, Jensen et al.: "Increased cyclooxygenase-2 expression and prostaglandin E2 production in pressurized renal medullary interstitial cells." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 299, Issue 3, pp. R823-31, 2010 (PubMed).

Chen, Gallup, Nikulina et al.: "Cigarette smoke induces epidermal growth factor receptor-dependent redistribution of apical MUC1 and junctional beta-catenin in polarized human airway epithelial cells." in: The American journal of pathology, Vol. 177, Issue 3, pp. 1255-64, 2010 (PubMed).

Khalaj, Peirovi, Khodagholi et al.: "Acute 17β-estradiol pretreatment protects against abdominal aortic occlusion induced spinal cord ischemic-reperfusion injury." in: Neurochemical research, Vol. 36, Issue 2, pp. 268-80, 2011 (PubMed).

Ji, Yuan, Liu et al.: "Developing multidrug-resistant cells and exploring correlation between BCRP/ABCG2 over-expression and DNA methyltransferase." in: Acta biochimica et biophysica Sinica, Vol. 42, Issue 12, pp. 854-62, 2010 (PubMed).

Horiguchi, Kouki, Fujiwara et al.: "The extracellular matrix component laminin promotes gap junction formation in the rat anterior pituitary gland." in: The Journal of endocrinology, Vol. 208, Issue 3, pp. 225-32, 2011 (PubMed).

Schusser, Reuter, von der Malsburg et al.: "Mx is dispensable for interferon-mediated resistance of chicken cells against influenza A virus." in: Journal of virology, Vol. 85, Issue 16, pp. 8307-15, 2011 (PubMed).

Chitalia, Murikipudi, Indolfi et al.: "Matrix-embedded endothelial cells are protected from the uremic milieu." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 26, Issue 12, pp. 3858-65, 2011 (PubMed).

Wang, Ding, Sun et al.: "Targeting of colorectal cancer growth, metastasis, and anti-apoptosis in BALB/c nude mice via APRIL siRNA." in: Molecular and cellular biochemistry, 2011 (PubMed).

Ilmiawati, Horiguchi, Fujiwara et al.: "Matrix metalloproteinase-9 expression in folliculostellate cells of rat anterior pituitary gland." in: The Journal of endocrinology, 2011 (PubMed).

Yoshioka, Yamamoto, Hanaoka et al.: "In vivo therapeutic effect of CDH3/P-cadherin-targeting radioimmunotherapy." in: Cancer immunology, immunotherapy : CII, 2012 (PubMed).

Horiguchi, Ilmiawati, Fujiwara et al.: "Expression of chemokine CXCL12 and its receptor CXCR4 in folliculostellate (FS) cells of the rat anterior pituitary gland: the CXCL12/CXCR4 axis induces interconnection of FS cells." in: Endocrinology, Vol. 153, Issue 4, pp. 1717-24, 2012 (PubMed).