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Actin, beta (ACTB) antibody

Antigen

Actin, beta (ACTB)

Synonyms
PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A19 ... show more
Clonality Monoclonal (B11V08)
Host
Alternatives

Mouse

Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus), Pig (Porcine), Sheep (Ovine), Chicken, Rabbit, Cow (Bovine)

Application
Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
9 references available
Catalog no. ABIN223292
Quantity 100 ug
Price 295.00 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information

Characteristics 100 ug mouse anti-beta-Actin monoclonal antibody. Reacts with human/mouse/rat/pig/sheep/chicken/rabbit/bovine. Tested in WB/IF/IHC.
Alternative name Beta-Actin
Gene ID 60
Immunogen Synthetic peptide corresponding to the N-terminus of human beta-Actin.
Format Purified
Isotype IgG2a  (Matching secondary antibodies)
Clone B11V08
Description Actin is expressed in all eukaryotic cells and is the major component of the cytoskeleton. At least six types of actin are present in mammalian tissues and fall into three classes. Alpha actin expression is limited to various types of muscle and it regulates contactile potentials for the muscle cells, whereas beta and gamma actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility.
Specificity The antibody recognizes 44 kDa beta-Actin in samples from human, mouse, rat, pig, sheep, chicken, rabbit, bovine origins.
Synonyms PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1

Application Details

Application Notes The antibody can be used for Western blotting (0.5-2 ug/ml), immunofluorescence (5-10 ug/ml) and immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually.
Buffer 100 ug (0. 5 mg/ml) Protein G affinity purified mouse anti-beta-Actin monoclonal antibody in phosphate-buffered saline (PBS) containing 0.02% thimerosal.
Storage Store at -20 o C. For long term storage, aliquot and freeze at
Restrictions For Research Use only

Publications

Publications Fullerton, Hakimuddin, Bakovic: "Developmental and metabolic effects of disruption of the mouse CTP:phosphoethanolamine cytidylyltransferase gene (Pcyt2)." in: Molecular and cellular biology, Vol. 27, Issue 9, pp. 3327-36, 2007 (PubMed).

Weng, Chau, Hsieh et al.: "Lucidenic acid inhibits PMA-induced invasion of human hepatoma cells through inactivating MAPK/ERK signal transduction pathway and reducing binding activities of NF-kappaB and AP-1." in: Carcinogenesis, Vol. 29, Issue 1, pp. 147-56, 2008 (PubMed).

Harada, Itasaka, Kizaka-Kondoh et al.: "The Akt/mTOR pathway assures the synthesis of HIF-1alpha protein in a glucose- and reoxygenation-dependent manner in irradiated tumors." in: The Journal of biological chemistry, Vol. 284, Issue 8, pp. 5332-42, 2009 (PubMed).

Ellis, Gattoni-Celli, Mancia et al.: "The vitamin D3 transcriptomic response in skin cells derived from the Atlantic bottlenose dolphin." in: Developmental and comparative immunology, Vol. 33, Issue 8, pp. 901-12, 2009 (PubMed).

Liu, Song, Wang et al.: "Effect of chronic intermittent hypoxia on biological behavior and hypoxic-associated gene expression in lung cancer cells." in: Journal of cellular biochemistry, 2010 (PubMed).

Khalaj, Peirovi, Khodagholi et al.: "Acute 17β-estradiol pretreatment protects against abdominal aortic occlusion induced spinal cord ischemic-reperfusion injury." in: Neurochemical research, Vol. 36, Issue 2, pp. 268-80, 2011 (PubMed).

Wang, Ding, Sun et al.: "Targeting of colorectal cancer growth, metastasis, and anti-apoptosis in BALB/c nude mice via APRIL siRNA." in: Molecular and cellular biochemistry, 2011 (PubMed).

Ilmiawati, Horiguchi, Fujiwara et al.: "Matrix metalloproteinase-9 expression in folliculostellate cells of rat anterior pituitary gland." in: The Journal of endocrinology, 2011 (PubMed).

Yoshioka, Yamamoto, Hanaoka et al.: "In vivo therapeutic effect of CDH3/P-cadherin-targeting radioimmunotherapy." in: Cancer immunology, immunotherapy : CII, 2012 (PubMed).

Alternatives

Alternatives for antigen "Actin, beta (ACTB)", type "Antibodies"
Hosts Rabbit (35), Mouse (31), Chicken (2)
Reactivities Human (56), Rat (Rattus) (25), Mouse (Murine) (23), Chicken (10), Rabbit (7), Cow (Bovine) (6), Pig (Porcine) (5), Sheep (Ovine) (4), Cat (Feline) (3), Dog (Canine) (3), Guinea Pig (3), Hamster (3), Monkey (3), Carp (2), Fish (2), Fruit Fly (Drosophila melanogaster) (2), Goat (2), Primate (1)
Applications Western Blotting (WB) (66), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (20), Immunofluorescence (IF) (16), ELISA (15), Enzyme Immunoassay (EIA) (14), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunohistochemistry (IHC) (5), Immunoprecipitation (IP) (5), Dot Blot (Dot) (4), Immunocytochemistry (ICC) (4), Flow Cytometry (FACS) (2), ELISA (Detection) (1)
Conjugates HRP (2), Biotin (1)
Epitopes N-Term (7), C-Term (1)