Actin, beta (ACTB) antibody

Antigen: Actin, beta (ACTB)

Synonyms: PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1

Clonality: Monoclonal (B11V08)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Pig (Porcine), Sheep (Ovine), Chicken, Rabbit, Cow (Bovine)

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN223292

Additional Information

Characteristics: 100 ug mouse anti-beta-Actin monoclonal antibody. Reacts with human/mouse/rat/pig/sheep/chicken/rabbit/bovine. Tested in WB/IF/IHC.

Alternative name: Beta-Actin

Gene ID: 60

Immunogen: Synthetic peptide corresponding to the N-terminus of human beta-Actin.

Format: Purified

Isotype: IgG2a  (Matching secondary antibodies)

Clone: B11V08

Description: Actin is expressed in all eukaryotic cells and is the major component of the cytoskeleton. At least six types of actin are present in mammalian tissues and fall into three classes. Alpha actin expression is limited to various types of muscle and it regulates contactile potentials for the muscle cells, whereas beta and gamma actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility.

Specificity: The antibody recognizes 44 kDa beta-Actin in samples from human, mouse, rat, pig, sheep, chicken, rabbit, bovine origins.

Synonyms: PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1

Application Details

Application Notes: The antibody can be used for Western blotting (0.5-2 ug/ml), immunofluorescence (5-10 ug/ml) and immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually.

Buffer: 100 ug (0. 5 mg/ml) Protein G affinity purified mouse anti-beta-Actin monoclonal antibody in phosphate-buffered saline (PBS) containing 0.02% thimerosal.

Storage: Store at -20 o C. For long term storage, aliquot and freeze at

Restrictions: For Research Use only

Publications

Publications: Fullerton, Hakimuddin, Bakovic: "Developmental and metabolic effects of disruption of the mouse CTP:phosphoethanolamine cytidylyltransferase gene (Pcyt2)." in: Molecular and cellular biology, Vol. 27, Issue 9, pp. 3327-36, 2007 (PubMed).

Weng, Chau, Hsieh et al.: "Lucidenic acid inhibits PMA-induced invasion of human hepatoma cells through inactivating MAPK/ERK signal transduction pathway and reducing binding activities of NF-kappaB and AP-1." in: Carcinogenesis, Vol. 29, Issue 1, pp. 147-56, 2008 (PubMed).

Harada, Itasaka, Kizaka-Kondoh et al.: "The Akt/mTOR pathway assures the synthesis of HIF-1alpha protein in a glucose- and reoxygenation-dependent manner in irradiated tumors." in: The Journal of biological chemistry, Vol. 284, Issue 8, pp. 5332-42, 2009 (PubMed).

Ellis, Gattoni-Celli, Mancia et al.: "The vitamin D3 transcriptomic response in skin cells derived from the Atlantic bottlenose dolphin." in: Developmental and comparative immunology, Vol. 33, Issue 8, pp. 901-12, 2009 (PubMed).

Liu, Song, Wang et al.: "Effect of chronic intermittent hypoxia on biological behavior and hypoxic-associated gene expression in lung cancer cells." in: Journal of cellular biochemistry, 2010 (PubMed).

Khalaj, Peirovi, Khodagholi et al.: "Acute 17β-estradiol pretreatment protects against abdominal aortic occlusion induced spinal cord ischemic-reperfusion injury." in: Neurochemical research, Vol. 36, Issue 2, pp. 268-80, 2011 (PubMed).

Wang, Ding, Sun et al.: "Targeting of colorectal cancer growth, metastasis, and anti-apoptosis in BALB/c nude mice via APRIL siRNA." in: Molecular and cellular biochemistry, 2011 (PubMed).

Ilmiawati, Horiguchi, Fujiwara et al.: "Matrix metalloproteinase-9 expression in folliculostellate cells of rat anterior pituitary gland." in: The Journal of endocrinology, 2011 (PubMed).

Yoshioka, Yamamoto, Hanaoka et al.: "In vivo therapeutic effect of CDH3/P-cadherin-targeting radioimmunotherapy." in: Cancer immunology, immunotherapy : CII, 2012 (PubMed).