Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) antibody

Antigen: Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1)

Synonyms: ARO, ARO1, CPV1, CYAR, CYP19, MGC104309, P-450AROM, Cyp19, Cyp19a, p450arom, Aromatase, p450arom-A, cyp19a, MGC84663, P450 arom, P450AROM

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus), Cow (Bovine), Pig (Porcine), Chicken

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN223293

Additional Information

Alternative name: Aromatase

Gene ID: 1588

Immunogen: Synthetic peptide surrounding amino acid 385 of human Aromatase

Format: Liquid

Isotype: IgG

Description: Aromatase is a key enzyme in steroidogenesis and plays an important role in sexual differentiation, fertility, and carcinogenesis. Many environmental chemicals may influence aromatase activity and thereby disrupt endocrine function.

Application Details

Application Notes: The antibody can be used for Western blotting (0.5-4 ug/ml). Based on researcher's feedback, the antibody can also be used in immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control.

Handling Advice: The antibody solution should be gently mixed before use.

Concentration: 0.2 mg/mL

Purification: Affinity purified

Buffer: phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Preservative: Thimerosal

Storage: -20 °C

Expiry Date: 12 months

Research Area: Enzymes

Restrictions: For Research Use only

Images

Western blot analysis of Aromatase in human brain cell lysate.

Publications

Product: Sun, Yan, Jacobson et al.: "Contribution of epoxyeicosatrienoic acids to flow-induced dilation in arteries of male ERalpha knockout mice: role of aromatase." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 293, Issue 3, pp. R1239-46, 2007 (PubMed).

Bukulmez, Hardy, Carr et al.: "Inflammatory status influences aromatase and steroid receptor expression in endometriosis." in: Endocrinology, Vol. 149, Issue 3, pp. 1190-204, 2008 (PubMed).

Hirayama, Sawai, Moriyasu et al.: "Excess estrogen sulfoconjugation as the possible cause for a poor sign of parturition in pregnant cows carrying somatic cell clone fetuses." in: Reproduction (Cambridge, England), Vol. 136, Issue 5, pp. 639-47, 2008 (PubMed).

Yonezawa, Higashi, Yoshioka et al.: "Distribution of aromatase and sex steroid receptors in the baculum during the rat life cycle: effects of estrogen during the early development of the baculum." in: Biology of reproduction, Vol. 85, Issue 1, pp. 105-12, 2011 (PubMed).