Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) antibody

Details for Product No. ABIN223293, Supplier: Log in to see
Antigen
  • CYP19
  • CYP19A1
  • ARO
  • ARO1
  • CPV1
  • CYAR
  • CYPXIX
  • P-450AROM
  • Aromatase
  • Cyp19
  • Cyp19a
  • p450arom
  • CYP19P1
  • Ar
  • ArKO
  • Int-5
  • Int5
  • P450AROM
  • P450 arom
  • aro
  • cyp19
  • cyp19a
  • p450arom-A
  • aromatase
  • cytochrome P450 aromatase
  • cytochrome P450 19A1
  • cytochrome P450 19A3
  • cytochrome P450, family 17, subfamily A, polypeptide 1
  • cytochrome P450, family 19
  • cytochrome P450, family 19, subfamily A, polypeptide 1
  • cytochrome P450, family 19, subfamily a, polypeptide 1
  • SAV_2383
  • pfcyp19b-tstr
  • CYP19A1
  • CYP19A3
  • CYP19
  • Cyp19a1
  • cyp19a1
Alternatives
anti-Human Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 antibody for Immunofluorescence (fixed cells)
Reactivity
Chicken, Cow (Bovine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
150
67
47
23
13
11
10
9
9
7
7
4
4
1
1
1
1
1
1
1
1
1
Host
Rabbit
106
30
20
Clonality
Polyclonal
Conjugate
Un-conjugated
6
5
5
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
119
40
39
39
26
25
14
14
5
4
2
1
Options
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Available images

Immunogen Synthetic peptide surrounding amino acid 385 of human Aromatase
Blocking Peptide Blocking peptide for this product available: ABIN412600
Isotype IgG
Specificity A 55 kDa band can be detected, corresponding to the expected molecular weight of Aromatase.
Alternative Name Aromatase (CYP19A1 Antibody Abstract)
Background Aromatase is a key enzyme in steroidogenesis and plays an important role in sexual differentiation, fertility, and carcinogenesis. Many environmental chemicals may influence aromatase activity and thereby disrupt endocrine function.
Synonyms: 25147 , Cyp19a1, Aromatase , Cyp19 , p450arom , ARO1, ARO 1, CPV, CYP19A1 , MGC104309
Gene ID 1588
UniProt P11511
Research Area Cancer, Endocrine system, Enzymes
Pathways Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis
Application Notes Western blotting (0.5-4 μg/mL), Immunohistochemistry (10-20 μg/mL). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody should recognize aromatase in samples from human, mouse, rat, bovine, porcine, pig, sheep, rabbit, horse and chicken origins. A 55 kDa band can be detected, corresponding to the expected molecular weight of Aromatase. An additional 35 kDa band (believe to be the cleavage product of Aromatase) can also be detected in Jurkat cells.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) affinity purified rabbit anti-Aromatase polyclonal antibody in phosphate-buffered saline (PBS) containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) antibody (ABIN223293) Western blot analysis of Aromatase in human brain cell lysate.
Product cited in: El Hajj Chehadeh, Dreumont, Willekens, Canabady-Rochelle, Jeannesson, Alberto, Daval, Guéant, Leininger-Muller: "Early methyl donor deficiency alters cAMP signaling pathway and neurosteroidogenesis in the cerebellum of female rat pups." in: American journal of physiology. Endocrinology and metabolism, Vol. 307, Issue 11, pp. E1009-19, 2014 (PubMed).

Yonezawa, Higashi, Yoshioka, Mutoh: "Distribution of aromatase and sex steroid receptors in the baculum during the rat life cycle: effects of estrogen during the early development of the baculum." in: Biology of reproduction, Vol. 85, Issue 1, pp. 105-12, 2011 (PubMed).

Bukulmez, Hardy, Carr, Word, Mendelson: "Inflammatory status influences aromatase and steroid receptor expression in endometriosis." in: Endocrinology, Vol. 149, Issue 3, pp. 1190-204, 2008 (PubMed).

Hirayama, Sawai, Moriyasu, Hirayama, Goto, Kaneko, Miyamoto, Ushizawa, Takahashi, Minamihashi: "Excess estrogen sulfoconjugation as the possible cause for a poor sign of parturition in pregnant cows carrying somatic cell clone fetuses." in: Reproduction (Cambridge, England), Vol. 136, Issue 5, pp. 639-47, 2008 (PubMed).

Sun, Yan, Jacobson, Jiang, Carroll, Huang: "Contribution of epoxyeicosatrienoic acids to flow-induced dilation in arteries of male ERalpha knockout mice: role of aromatase." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 293, Issue 3, pp. R1239-46, 2007 (PubMed).