Histone Deacetylase 5 (HDAC5) antibody

Details for Product No. ABIN223300, Supplier: Log in to see
Antigen
  • HDAC5
  • DKFZp459O115
  • HD5
  • NY-CO-9
  • AI426555
  • Hdac4
  • mHDA1
  • mKIAA0600
  • ATHDA5
  • HDA5
  • MAF19.7
  • MAF19_7
  • histone deacetylase 5
  • Hd6
  • Hdac5
  • Sfc6
  • mHDA2
  • histone deacetylase 5
  • histone deacetylase 6
  • HDAC5
  • Hdac5
  • HDA05
  • Hdac6
Alternatives
anti-Rat (Rattus) Histone Deacetylase 5 antibody for ELISA
Reactivity
Human, Mouse (Murine), Rat (Rattus)
176
89
49
13
10
6
4
4
4
3
2
2
1
1
1
Host
Rabbit
161
16
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
3
3
3
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
161
93
70
38
29
20
15
6
3
3
2
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen Synthetic peptide surrounding amino acid 575 of human HDAC-5
Blocking Peptide Blocking peptide for this product available: ABIN412768
Isotype IgG
Specificity The antibody detects ~124 kDa HDAC5 with human and mouse samples, and in a lesser extent with rat samples.
It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 6, 7, 8, 9,10, and 11.
Cross-Reactivity (Details) Does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 6, 7, 8, 9,10, and 11.
Purification Affinity purified
Alternative Name HDAC5 (HDAC5 Antibody Abstract)
Background Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence. Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.
Synonyms: HD5 , FLJ90614 , KIAA0600 , NY-CO-9
Gene ID 10014
UniProt Q9UQL6
Research Area Cardiovascular, Hypertrophy, Wnt, DNA/RNA, Transcription Factors
Pathways Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Monocarboxylic Acid Catabolic Process
Application Notes Western blotting (0.5-4 μg/mL), Immunoprecipitation (20 μg/mL), and Immunohistochemistry (20 μg/mL). However, the optimal conditions should be determined individually. The antibody detects ~124 kDa HDAC5 with human and mouse samples, and in a lesser extent with rat samples. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 6, 7, 8, 9,10, and 11.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) affinity purified rabbit anti-HDAC5 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Histone Deacetylase 5 (HDAC5) antibody (ABIN223300) Western blot analysis of HDAC5 expression in Jurkat cell lysate.
Product cited in: Cao, Li, Zhu, Shen, Han, Zhang, Yu, Wang, Wu, Chen, Sun, Tang, Zhao, Qiao, Hou, Mao: "The antiparasitic clioquinol induces apoptosis in leukemia and myeloma cells by inhibiting histone deacetylase activity." in: The Journal of biological chemistry, Vol. 288, Issue 47, pp. 34181-9, 2013 (PubMed).

Yamaguchi, Chakraborty, Pasek, Molkentin, Meissner: "Dysfunctional ryanodine receptor and cardiac hypertrophy: role of signaling molecules." in: American journal of physiology. Heart and circulatory physiology, Vol. 300, Issue 6, pp. H2187-95, 2011 (PubMed).