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Histone Deacetylase 5 (HDAC5) antibody

Details for Product No. ABIN223300, Supplier: Login to see
Antigen
  • HDAC5
  • DKFZp459O115
  • HD5
  • NY-CO-9
  • AI426555
  • Hdac4
  • mHDA1
  • mKIAA0600
  • ATHDA5
  • HDA5
  • MAF19.7
  • MAF19_7
  • histone deacetylase 5
  • Hd6
  • Hdac5
  • Sfc6
  • mHDA2
Reactivity
Human, Mouse (Murine), Rat (Rattus)
162
71
41
9
6
2
Host
Rabbit
154
8
Clonality
Polyclonal
Conjugate
Un-conjugated
3
2
2
2
2
2
2
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
128
92
63
25
20
17
7
3
3
2
2
1
Supplier
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Immunogen Synthetic peptide surrounding amino acid 575 of human HDAC-5
Blocking Peptide Blocking peptide for this product available: ABIN412768
Isotype IgG
Specificity The antibody detects ~124 kDa HDAC5 with human and mouse samples, and in a lesser extent with rat samples.
It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 6, 7, 8, 9,10, and 11.
Cross-Reactivity (Details) Does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 6, 7, 8, 9,10, and 11.
Purification Affinity purified
Alternative Name HDAC5 (HDAC5 Antibody Abstract)
Background Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence. Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.
Synonyms: HD5 , FLJ90614 , KIAA0600 , NY-CO-9
Gene ID 10014
UniProt Q9UQL6
Research Area Cardiovascular, Hypertrophy, Wnt, DNA/RNA, Transcription Factors
Pathways
Application Notes Western blotting (0.5-4 μg/mL), Immunoprecipitation (20 μg/mL), and Immunohistochemistry (20 μg/mL).
However, the optimal conditions should be determined individually.
Comment

Country of Animal Origin: China

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) affinity purified rabbit anti-HDAC5 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Histone Deacetylase 5 (HDAC5) antibody (ABIN223300) Western blot analysis of HDAC5 expression in Jurkat cell lysate.
Product cited in: Cao, Li, Zhu et al.: "The antiparasitic clioquinol induces apoptosis in leukemia and myeloma cells by inhibiting histone deacetylase activity." in: The Journal of biological chemistry, Vol. 288, Issue 47, pp. 34181-9, 2013 (PubMed).

Yamaguchi, Chakraborty, Pasek et al.: "Dysfunctional ryanodine receptor and cardiac hypertrophy: role of signaling molecules." in: American journal of physiology. Heart and circulatory physiology, Vol. 300, Issue 6, pp. H2187-95, 2011 (PubMed).