beta Actin antibody (Actin, beta)

Details for Product anti-ACTB Antibody No. ABIN223341, Supplier: Log in to see
Antigen
  • beta-actin
  • ps1tp5bp1
  • LOC563571
  • ACTB
  • A26C1A
  • A26C1B
  • act-2
  • actb
  • B-actin
  • LOC100136013
  • LOC100174883
  • LOC100304816
  • A
  • ACT5C
  • Ac5C
  • Act
  • Act-5C
  • Act5
  • Act5c
  • Actin
  • Actin/BAP47
  • Actin5C
  • BAP47
  • Bap47
  • CG4027
  • Dmel\\CG4027
  • M32055
  • T11
  • act
  • act 5C
  • act5C
  • actin
  • actin5C
  • anon-EST:fe2D2
  • beta-actin/Bap47
  • cyt5C
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • Bact
  • BRWS1
  • PS1TP5BP1
  • ACT-5
  • Actx
  • E430023M04Rik
  • ACTA1
  • Beta-actin
  • B-ACTZF
  • actba
  • bact
  • bactin1
  • bactzf
  • wu:fd18f05
  • POTE2alpha
  • POTEACTIN
  • actin, beta
  • similar to beta-cytoplasmic actin2
  • cytoplasmic actin OlCA1
  • actin beta
  • beta-actin
  • Actin 5C
  • actin, beta 1
  • actin, gamma 1
  • beta actin
  • POTE ankyrin domain family, member F
  • actb
  • LOC563571
  • ACTB
  • LOC100136013
  • LOC100174883
  • LOC100304816
  • Act5C
  • Actb
  • actb1
  • ACTG1
  • LOC396797
  • LOC100350495
  • LOC100136352
  • POTEF
Alternatives
anti-Cow (Bovine) beta Actin antibody for Immunocytochemistry
Reactivity
Human, Mouse (Murine), Rat (Rattus)
574
349
299
104
82
76
60
59
45
42
42
37
32
28
22
16
16
14
13
13
9
9
8
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5
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
358
235
16
8
1
1
Clonality
Polyclonal
Conjugate
This beta Actin antibody is un-conjugated
32
27
18
6
6
5
5
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4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
550
228
157
137
122
100
59
46
19
14
13
12
10
9
9
2
2
2
2
2
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Synthetic peptide corresponding to residues around amino acid 10 of human Actin
Blocking Peptide Blocking peptide for this product available: ABIN412613
Isotype IgG
Specificity The antibody recognizes 45 kDa beta-Actin in samples from human and mouse origins.
Reactivity to other species has not been tested.
Purification Affinity purified
Alternative Name beta-Actin (ACTB Antibody Abstract)
Background Actin is expressed in all eukaryotic cells and is the major component of the cytoskeleton. At least six types of actin are present in mammalian tissues and fall into three classes. Alpha actin expression is limited to various types of muscle and it regulates contactile potentials for the muscle cells, whereas beta and gamma actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility.
Synonyms: PS1TP5BP1 , ACTB, Beta-actin , b-actin
Gene ID 60
UniProt P02570
Pathways Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
Application Notes Western blotting (0.5-2 μg/mL). However, the optimal concentrations should be determined individually. The antibody recognizes 45 kDa beta-Actin in samples from human and mouse origins. Reactivity to other species has not been tested.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) protein A purified rabbit anti-β-Actin polyclonal antibody in phosphate-buffered saline (PBS) containing 0.5 % BSA, 30 % glycerol, and 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Actin, beta (ACTB) antibody (ABIN223341) Western blot analysis of -Actin in Jurkat cell lysate (left lane) and mouse intestine...
Product cited in: Mizutani, Inoue, Omori, Ito, Tamiya-Koizumi, Takagi, Kojima, Nakamura, Iwaki, Nakatochi, Suzuki, Nozawa, Murate: "Increased acid ceramidase expression depends on upregulation of androgen-dependent deubiquitinases, USP2, in a human prostate cancer cell line, LNCaP." in: Journal of biochemistry, 2015 (PubMed).

Alarab, Kufaishi, Lye, Drutz, Shynlova: "Expression of extracellular matrix-remodeling proteins is altered in vaginal tissue of premenopausal women with severe pelvic organ prolapse." in: Reproductive sciences (Thousand Oaks, Calif.), Vol. 21, Issue 6, pp. 704-15, 2014 (PubMed).

Saeki, Inui, Fujioka, Fukuda, Nomura, Nakamura, Park, Sato, Kanamoto: "Staurosporine synergistically potentiates the deoxycholate-mediated induction of COX-2 expression." in: Physiological reports, Vol. 2, Issue 8, 2014 (PubMed).

Mishima, Maeshima, Miya, Sakurai, Ikeuchi, Hiromura, Nojima: "Involvement of N-type Ca(2+) channels in the fibrotic process of the kidney in rats." in: American journal of physiology. Renal physiology, Vol. 304, Issue 6, pp. F665-73, 2013 (PubMed).

Ding, Chang, Shen, Tai: "Murine tyrosinase Inhibitors from Cynanchum bungei and evaluation of in vitro and in vivo depigmenting activity." in: Experimental dermatology, 2011 (PubMed).

Chen, Ding, Ding, Xiang, Li, Ghosh, Schurig, Sriranganathan, Boyle, He: "Proinflammatory caspase-2-mediated macrophage cell death induced by a rough attenuated Brucella suis strain." in: Infection and immunity, Vol. 79, Issue 6, pp. 2460-9, 2011 (PubMed).

Chang, Lin: "Inhibitory effect of danazol on melanogenesis in mouse B16 melanoma cells." in: Archives of pharmacal research, Vol. 33, Issue 12, pp. 1959-65, 2010 (PubMed).

Komagata, Nakajima, Takagi, Mohri, Taniya, Yokoi: "Human CYP24 catalyzing the inactivation of calcitriol is post-transcriptionally regulated by miR-125b." in: Molecular pharmacology, Vol. 76, Issue 4, pp. 702-9, 2009 (PubMed).

Tewari, Kalkunte, Murray, Sharma: "The water channel aquaporin 1 is a novel molecular target of polychlorinated biphenyls for in utero anomalies." in: The Journal of biological chemistry, Vol. 284, Issue 22, pp. 15224-32, 2009 (PubMed).

Wang, Allen, May, Lightfoot, Houchen, Huycke: "Enterococcus faecalis induces aneuploidy and tetraploidy in colonic epithelial cells through a bystander effect." in: Cancer research, Vol. 68, Issue 23, pp. 9909-17, 2008 (PubMed).

Fullerton, Hakimuddin, Bakovic: "Developmental and metabolic effects of disruption of the mouse CTP:phosphoethanolamine cytidylyltransferase gene (Pcyt2)." in: Molecular and cellular biology, Vol. 27, Issue 9, pp. 3327-36, 2007 (PubMed).

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