Low Density Lipoprotein Receptor-Related Protein (LRP) antibody

Antigen: Low Density Lipoprotein Receptor-Related Protein (LRP)

Clonality: Monoclonal (1A12)

Host: Mouse

Reactivity: Mouse (Murine), Human, Mouse (Murine)

Application: Western Blotting (WB)

Catalog no.: ABIN223471

Additional Information

Alternative name: LRP-5/6

Gene ID: 4041

Immunogen: Synthetic peptide derived from human LRP-6. These residues are completely conserved between human and mouse and between LRP-5 and LRP-6.

Format: Purified

Isotype: IgG2b

Clone: 1A12

Description: 100 ug purified mouse anti-LRP-5/6 (LDL-related protein) IgG2b (clone 1A12). Reacts with human/mouse. Tested in WB. Synonyms: LRP Monoclonal Antibody, Anti-LRP antibody, LRP antibody, Anti-LRP, LRP, LRP-5, LRP-6, LDL-related protein, LDL receptor, Axin, beta-catenin Synonyms: LRP Monoclonal Antibody, Anti-LRP antibody, LRP antibody, Anti-LRP, LRP, LRP-5, LRP-6, LDL-related protein, LDL receptor, Axin, beta-catenin

Application Details

Application Notes: Western blot analysis (1-2 ug/ml). However, the optimal conditions should be determined individually.

Buffer: 100 ug (1 mg/ml) purified antibody in 10 mM Tris-glycine (pH 7.0), 0.15 M NaCl, 25% glycerol, 0.5% BSA, and 0.01% sodium azide.

Storage: Store at -20 o C. For long-term storage, aliquot and refreeze at -70 o C. Avoid repeated freeze/defrost cycles Shelf Life: 12 Months

Restrictions: For Research Use only

Publications

Product: Caricasole, Ferraro, Iacovelli et al.: "Functional characterization of WNT7A signaling in PC12 cells: interaction with A FZD5 x LRP6 receptor complex and modulation by Dickkopf proteins." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37024-31, 2003 (PubMed).

Berasain, García-Trevijano, Castillo et al.: "Novel role for amphiregulin in protection from liver injury." in: The Journal of biological chemistry, Vol. 280, Issue 19, pp. 19012-20, 2005 (PubMed).

Cappuccio, Calderone, Busceti et al.: "Induction of Dickkopf-1, a negative modulator of the Wnt pathway, is required for the development of ischemic neuronal death." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 10, pp. 2647-57, 2005 (PubMed).

Ai, Holmen, Van Hul et al.: "Reduced affinity to and inhibition by DKK1 form a common mechanism by which high bone mass-associated missense mutations in LRP5 affect canonical Wnt signaling." in: Molecular and cellular biology, Vol. 25, Issue 12, pp. 4946-55, 2005 (PubMed).

Rajamannan, Subramaniam, Caira et al.: "Atorvastatin inhibits hypercholesterolemia-induced calcification in the aortic valves via the Lrp5 receptor pathway." in: Circulation, Vol. 112, Issue 9 Suppl, pp. I229-34, 2005 (PubMed).

Caira, Stock, Gleason et al.: "Human degenerative valve disease is associated with up-regulation of low-density lipoprotein receptor-related protein 5 receptor-mediated bone formation." in: Journal of the American College of Cardiology, Vol. 47, Issue 8, pp. 1707-12, 2006 (PubMed).

Caverzasio, Manen: "Essential role of Wnt3a-mediated activation of mitogen-activated protein kinase p38 for the stimulation of alkaline phosphatase activity and matrix mineralization in C3H10T1/2 mesenchymal cells." in: Endocrinology, Vol. 148, Issue 11, pp. 5323-30, 2007 (PubMed).