Myc Tag antibody

Details for Product No. ABIN223509, Supplier: Log in to see
Antigen
Reactivity
Tag
212
36
14
10
6
5
3
1
1
Host
Rabbit
161
59
17
16
3
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
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23
14
9
5
4
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
215
116
77
52
48
34
22
19
19
7
6
5
5
5
5
4
3
3
3
2
1
1
1
1
1
1
1
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Immunogen Synthetic peptide corresponding to residues surrounding amino acid 413 of human c-Myc
Blocking Peptide Blocking peptide for this product available: ABIN412841
Sequence EQKLISEEDL
Isotype IgG
Specificity The antibody detects Myc-Tag proteins overexpressed in cells.
It does not detect endogenous levels of the transcription factor c-Myc.
Purification Affinity purified
Alternative Name C-Myc Epitope Tag
Target Type Tag
Background Epitope tags are usually for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian systems.
Synonyms: MYC , C-MYC , c-Myc
Gene ID 4609
UniProt P01106
Research Area Transcription Factors
Application Notes Western blotting (1-2 μg/mL), immunoprecipitation (20-40 μg/mL), and immunocytochemistry (10-20 μg/mL). However, the optimal conditions should be determined individually. The antibody detects Myc-Tag proteins overexpressed in cells. It does not detect endogenous levels of the transcription factor c-Myc.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) peptide affinity purified rabbit anti-Myc-Tag polyclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
 image for anti-Myc Tag antibody (ABIN223509) anti-Myc Tag antibody
Product cited in: Kumar, Shum, Glaunsinger: "Importin alpha-mediated nuclear import of cytoplasmic poly(A) binding protein occurs as a direct consequence of cytoplasmic mRNA depletion." in: Molecular and cellular biology, Vol. 31, Issue 15, pp. 3113-25, 2011 (PubMed).

Lei, Shi, Basu, Huq, Routhier, Day, Jin: "Proteolytic processing of angiopoietin-like protein 4 by proprotein convertases modulates its inhibitory effects on lipoprotein lipase activity." in: The Journal of biological chemistry, Vol. 286, Issue 18, pp. 15747-56, 2011 (PubMed).

Liu, Afroza, Rader, Jin: "Angiopoietin-like protein 3 inhibits lipoprotein lipase activity through enhancing its cleavage by proprotein convertases." in: The Journal of biological chemistry, Vol. 285, Issue 36, pp. 27561-70, 2010 (PubMed).