His Tag antibody

Details for Product No. ABIN223514, Supplier: Log in to see
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reactivity
Tag
616
24
22
14
3
2
Host
Rabbit
435
183
27
23
4
4
3
1
Clonality
Polyclonal
Conjugate
Un-conjugated
59
45
36
15
14
12
11
10
10
10
10
9
8
8
8
8
7
7
6
5
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunochromatography (IC), Immunoprecipitation (IP), Western Blotting (WB)
554
386
166
107
99
77
69
26
12
11
9
7
7
6
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Immunogen 6xHis synthetic peptide
Blocking Peptide Blocking peptide for this product available: ABIN412778
Sequence HHHHHH
Isotype IgG
Specificity The antibody detects His-tagged recombinant proteins.
Purification Affinity purified
Alternative Name His-Tag
Target Type Tag
Background A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
Synonyms: His tag, HHHHHH tag
Research Area Tags/Labels
Application Notes Western blotting (1 μg/mL), immunoprecipitation (40 μg/mL), and immunocytochemistry (2 μg/mL). However, the optimal conditions should be determined individually. The antibody detects His-tagged recombinant proteins.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) peptide affinity purified rabbit anti-His-Tag polyclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-His Tag antibody (ABIN223514) anti-His Tag antibody
Product cited in: Yin, Johns, Lawrence, Xu, Reddi, Bishop, Varner, Fuster: "Lymphatic endothelial heparan sulfate deficiency results in altered growth responses to vascular endothelial growth factor-C (VEGF-C)." in: The Journal of biological chemistry, Vol. 286, Issue 17, pp. 14952-62, 2011 (PubMed).

Tsang, Felek, Krukonis: "Ail binding to fibronectin facilitates Yersinia pestis binding to host cells and Yop delivery." in: Infection and immunity, Vol. 78, Issue 8, pp. 3358-68, 2010 (PubMed).

Did you look for something else?