GFP antibody (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN223515, Supplier: Log in to see
Antigen
  • green fluorescent protein
  • gfp
Reactivity
Aequorea victoria
639
5
5
2
2
2
1
1
Host
Rabbit
328
170
80
41
16
9
8
2
Clonality
Polyclonal
Conjugate
This GFP antibody is un-conjugated
32
30
27
14
12
11
11
8
8
8
7
7
6
5
5
5
5
5
5
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
522
338
189
143
141
120
96
72
58
26
15
13
13
8
8
6
6
5
4
4
3
3
3
3
3
2
2
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen Recombinant EGFP
Isotype IgG
Specificity The antibody reacts with GFP, and its variants EGFP, RFP, YFP, and CFP, etc.
Purification Affinity purified
Alternative Name GFP
Background Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP can fuse with proteins of interest without interfering significantly with their assembly and function.
Synonyms: Green Fluorescent Protein
Gene ID 110612126
Research Area Tags/Labels
Application Notes Western blotting (0.5-4 μg/mL) and in immunoprecipitation (10-20 μg/mL). However, the optimal conditions should be determined individually. The antibody reacts with GFP, and its variants EGFP, RFP, YFP, and CFP, etc. Recombinant EGFP, RFP, YFP and CFP can be used as positive controls.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) affinity purified rabbit anti-GFP polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN223515) Western blot analysis of GFP antibody using recombinant RFP (dsRed, Lane 1 & 2), YFP ...
Product cited in: Maus, Jairaman, Stathopulos, Muik, Fahrner, Weidinger, Benson, Fuchs, Ehl, Romanin, Ikura, Prakriya, Feske: "Missense mutation in immunodeficient patients shows the multifunctional roles of coiled-coil domain 3 (CC3) in STIM1 activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 19, pp. 6206-11, 2015 (PubMed).

German, Mammoto, Jiang, Ingber, Mammoto: "Paxillin controls endothelial cell migration and tumor angiogenesis by altering neuropilin 2 expression." in: Journal of cell science, Vol. 127, Issue Pt 8, pp. 1672-83, 2014 (PubMed).

Hagen, Bayer, Rösch, Schindler: "The intraviral protein interaction network of hepatitis C virus." in: Molecular & cellular proteomics : MCP, Vol. 13, Issue 7, pp. 1676-89, 2014 (PubMed).

Hsu, Lin, Chang: "Synergetic regulation of translational reading-frame switch by ligand-responsive RNAs in mammalian cells." in: Nucleic acids research, Vol. 42, Issue 22, pp. 14070-82, 2014 (PubMed).

Martínez-Fábregas, Díaz-Moreno, González-Arzola, Janocha, Navarro, Hervás, Bernhardt, Velázquez-Campoy, Díaz-Quintana, De la Rosa: "Structural and functional analysis of novel human cytochrome C targets in apoptosis." in: Molecular & cellular proteomics : MCP, Vol. 13, Issue 6, pp. 1439-56, 2014 (PubMed).

Mellergaard, Skovbakke, Schneider, Lauridsen, Andresen, Jensen, Skov: "N-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24." in: The Journal of biological chemistry, Vol. 289, Issue 29, pp. 20078-91, 2014 (PubMed).

Martínez-Fábregas, Díaz-Moreno, González-Arzola, Janocha, Navarro, Hervás, Bernhardt, Díaz-Quintana, De la Rosa: "New Arabidopsis thaliana cytochrome c partners: a look into the elusive role of cytochrome c in programmed cell death in plants." in: Molecular & cellular proteomics : MCP, Vol. 12, Issue 12, pp. 3666-76, 2013 (PubMed).

Makovski, Yaffe, Shpungin, Nir: "Intronic promoter drives the BORIS-regulated expression of FerT in colon carcinoma cells." in: The Journal of biological chemistry, Vol. 287, Issue 9, pp. 6100-12, 2012 (PubMed).

Andresen, Skovbakke, Persson, Hagemann-Jensen, Hansen, Jensen, Skov: "2-deoxy D-glucose prevents cell surface expression of NKG2D ligands through inhibition of N-linked glycosylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 4, pp. 1847-55, 2012 (PubMed).

Bhat, Folimonova, Cole, Ballard, Lei, Watson, Sumner, Nelson: "Influence of host chloroplast proteins on Tobacco mosaic virus accumulation and intercellular movement." in: Plant physiology, Vol. 161, Issue 1, pp. 134-47, 2012 (PubMed).

Welman, Serrels, Brunton, Ditzel, Frame: "Two-color photoactivatable probe for selective tracking of proteins and cells." in: The Journal of biological chemistry, Vol. 285, Issue 15, pp. 11607-16, 2010 (PubMed).

Kock, Bulgakova, Knust, Sinning, Panneels: "Targeting of Drosophila rhodopsin requires helix 8 but not the distal C-terminus." in: PLoS ONE, Vol. 4, Issue 7, pp. e6101, 2009 (PubMed).

Creighton, Zhu, Alexeyev, Stevens: "Spectrin-anchored phosphodiesterase 4D4 restricts cAMP from disrupting microtubules and inducing endothelial cell gap formation." in: Journal of cell science, Vol. 121, Issue Pt 1, pp. 110-9, 2007 (PubMed).