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KC antibody

Details for Product No. ABIN223557, Supplier: Log in to see
Antigen
Reactivity
Mouse (Murine), Rat (Rattus)
10
3
2
Host
Rabbit
11
Clonality
Polyclonal
Conjugate
Un-conjugated
2
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
11
6
4
1
1
Options
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Immunogen E. coli-expressed rat KC
Isotype IgG
Purification Affinity purified
Background KC, a homolog of human and hamster gro/MGSA, is a 72-amino acid CXC chemokine originally cloned from rat macrophages and lung tissue. It is the mediator for recruitment and activation of netrophils in rat lung inflammation models. Expression of KC can be upregulated by LPS and IL-1β stimulation. IFN-g blocks LPS-induced expression of KC.
Synonyms: GRO alpha, GRO, chemokine (C-X-C), KC
Gene ID 206687
Application Notes Western blot analysis (0.5-4 μg/mL), Immunoprecipitation (3-5 μg/mL), and Immunohistochemistry (10-20 μg/mL). However, the optimal conditions should be determined individually.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) protein A purified rabbit anti-KC polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-KC antibody (ABIN223557) Western blot analysis of KC in transfected cells.
Product cited in: Leja, Kojalo, Frickauss, Bandere, Gavars, Boka: "Changing patterns of serological testing for celiac disease in Latvia." in: Journal of gastrointestinal and liver diseases : JGLD, Vol. 20, Issue 2, pp. 121-6, 2011 (PubMed).

Thaxton, Romero, Sharma: "TLR9 activation coupled to IL-10 deficiency induces adverse pregnancy outcomes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 2, pp. 1144-54, 2009 (PubMed).