CD207 Molecule, Langerin (CD207) antibody

Antigen: CD207 Molecule, Langerin (CD207)

Synonyms: CD207, CLEC4K

Clonality: Monoclonal (929F3-01)

Host: Rat

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN223760

Additional Information

Alternative name: CD207 / Langerin

Immunogen: recombinant human langerin

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG2a

Clone: 929F3-01

Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. Human Langerin is involved in the processing of mycobacterial glycolipids leading to antigen presentation to CD1a-restricted T cells. Human Langerin binds the gp120 glycoprotein of HIV.(Valladeau J et al, 2002 J Immunol, 168 : 782-792) (Douillard P et al, 2005 J Invest Dermatol, 125: 983-994)

Specificity: Human Langerin

Application Details

Application Notes: Usage Recommendation: This monoclonal antibody may be used between 5-20 ug/ml .

Purification: Purified

Buffer: 100 ug in 200ul Tris-NaCl pH 8

Storage: -20°C. KEEP CONTENTS STERILE: no preservative.

Research Area: CD Antigens

Restrictions: For Research Use only

Images

Immunofluorescence on mouse epidermal LC CD11c+

X20 : IHC on human skin with 929F3-HRP

X40 : IHC on human skin with 929F3-HRP

Publications

Dubrac, Stoitzner, Pirkebner et al.: "Peroxisome proliferator-activated receptor-alpha activation inhibits Langerhans cell function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 7, pp. 4362-72, 2007 (PubMed).

Zimmerli, Hauser: "Langerhans cells and lymph node dendritic cells express the tight junction component claudin-1." in: The Journal of investigative dermatology, Vol. 127, Issue 10, pp. 2381-90, 2007 (PubMed).

Stoecklinger, Grieshuber, Scheiblhofer et al.: "Epidermal langerhans cells are dispensable for humoral and cell-mediated immunity elicited by gene gun immunization." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 2, pp. 886-93, 2007 (PubMed).

Kaplan, Li, Jenison et al.: "Autocrine/paracrine TGFbeta1 is required for the development of epidermal Langerhans cells." in: The Journal of experimental medicine, Vol. 204, Issue 11, pp. 2545-52, 2007 (PubMed).

Poulin, Henri, de Bovis et al.: "The dermis contains langerin+ dendritic cells that develop and function independently of epidermal Langerhans cells." in: The Journal of experimental medicine, Vol. 204, Issue 13, pp. 3119-31, 2007 (PubMed).

Bursch, Wang, Igyarto et al.: "Identification of a novel population of Langerin+ dendritic cells." in: The Journal of experimental medicine, Vol. 204, Issue 13, pp. 3147-56, 2007 (PubMed).

Flacher, Douillard, Auit-Yahia et al.: "Expression of langerin/CD207 reveals dendritic cell heterogeneity between inbred mouse strains." in: Immunology, Vol. 123, Issue 3, pp. 339-47, 2008 (PubMed).

Stoitzner, Green, Jung et al.: "Inefficient presentation of tumor-derived antigen by tumor-infiltrating dendritic cells." in: Cancer immunology, immunotherapy : CII, Vol. 57, Issue 11, pp. 1665-73, 2008 (PubMed).

Shklovskaya, Roediger, Fazekas de St Groth: "Epidermal and dermal dendritic cells display differential activation and migratory behavior while sharing the ability to stimulate CD4+ T cell proliferation in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 1, pp. 418-30, 2008 (PubMed).

Tripp, Haid, Flacher et al.: "The lymph vessel network in mouse skin visualised with antibodies against the hyaluronan receptor LYVE-1." in: Immunobiology, Vol. 213, Issue 9-10, pp. 715-28, 2008 (PubMed).

Maddaluno, Verbrugge, Martinoli et al.: "The adhesion molecule L1 regulates transendothelial migration and trafficking of dendritic cells." in: The Journal of experimental medicine, Vol. 206, Issue 3, pp. 623-35, 2009 (PubMed).