FZD4 antibody (Extracellular Domain)
-
- Target See all FZD4 Antibodies
- FZD4 (Frizzled Family Receptor 4 (FZD4))
-
Binding Specificity
- Extracellular Domain
-
Reactivity
- Human, Mouse
-
Host
- Rat
-
Clonality
- Monoclonal
-
Conjugate
- This FZD4 antibody is un-conjugated
-
Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- Specificity
- Recognizes mouse Frizzled-4. Shows no cross-reactivity with rmFrizzled-1, -2, 3, -6, -7 -8 or rhFrizzled-5. Species cross-reactivity: Human. Detects Frizzled-4 by Flow Cytometry and Immunohistochemistry on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3. Also recognizes human Frizzled-4 by Flow Cytometry and Immunohistochemistry on HEK 293 cells.
- Purification
- Protein G purified
- Immunogen
- NS0-derived rmFrizzled-4 extracellular domain.
- Isotype
- IgG2a
- Top Product
- Discover our top product FZD4 Primary Antibody
-
-
- Application Notes
-
Approved: Flo (100 μg/mL), ICC (10 μg/mL), WB (1 - 2 μg/mL)
Usage: Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Flow Cytometry: 100 μg/mL. Add 10 μL to 1-2.5x10^5 cells. Total reaction volume should not exceeding 200 μL. Immunocytochemistry: 10 μg/mL in 4 % paraformaldehyde-fixed cells. Western Blot:1-2 μg/mL. The detection limit is ~5 ng/lane under non-reducing and reducing conditions. - Comment
-
Target Species of Antibody: Mouse
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Reconstitution
- Sterile PBS
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.2, 5 % trehalose.
- Handling Advice
- avoid freeze thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
-
- Target
- FZD4 (Frizzled Family Receptor 4 (FZD4))
- Alternative Name
- FZD4 / Frizzled 4 (FZD4 Products)
- Synonyms
- CG4626 antibody, DFz4 antibody, Dfz4 antibody, Dm Fz4 antibody, Dmel\\CG4626 antibody, Fz4 antibody, anon-WO0170980.10 antibody, anon-WO0170980.11 antibody, fz1 antibody, zg01 antibody, CD344 antibody, EVR1 antibody, FEVR antibody, FZD4S antibody, Fz-4 antibody, FzE4 antibody, GPCR antibody, hFz4 antibody, frizzled4 antibody, fz4 antibody, FZ-4 antibody, frizzled 4 antibody, frizzled class receptor 4 antibody, frizzled-4 antibody, frizzled class receptor 4 S homeolog antibody, fz4 antibody, fzd4 antibody, Tsp_10376 antibody, FZD4 antibody, Fzd4 antibody, fzd4.S antibody
- Background
-
Name/Gene ID: FZD4
Subfamily: Frizzled
Family: GPCR
Synonyms: FZD4, CD344, CD344 antigen, EVR1, Frizzled family receptor 4, Frizzled-4, Fz-4, Fz4, HFz4, Exudative vitreoretinopathy 1, FEVR, FZD4S, FzE4, Frizzled 4, Frizzled homolog 4, WNT receptor frizzled-4 - Gene ID
- 8322
- UniProt
- Q9ULV1
- Pathways
- WNT Signaling, Hormone Transport, Sensory Perception of Sound
-