IRS1 antibody (AA 1222-1235) (Biotin)
-
- Target See all IRS1 Antibodies
- IRS1 (Insulin Receptor Substrate 1 (IRS1))
-
Binding Specificity
- AA 1222-1235
-
Reactivity
- Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This IRS1 antibody is conjugated to Biotin
-
Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Specificity
- Recognizes rat IRS-1 at 160-185kD. Species cross-reactivity: Human, mouse and rat.
- Predicted Reactivity
- Percent identity by BLAST analysis: Rat (100%) Human, Gorilla, Mouse, Hamster, Rabbit (93%) Marmoset, Chicken (86%).
- Purification
- Protein A purified
- Immunogen
- Carboxy terminal 14 amino acid peptide ([C]YASINFQKQPEDRQ) of rat liver IRS-1. Percent identity by BLAST analysis: Rat (100%), Human, Gorilla, Mouse, Hamster, Rabbit (93%), Marmoset, Chicken (86%).
- Isotype
- IgG
- Top Product
- Discover our top product IRS1 Primary Antibody
-
-
- Application Notes
-
Approved: IP, WB (0.5 - 2 μg/mL)
Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-2 μg/mL detects IRS-1 in RIPA lysates from 3T3/A31 mouse fibroblasts. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-IRS-1 (0.5 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Previous lots detected IRS-1 in RIPA lysates of rat L6 myoblasts. In cells that contain high levels of IRS-1, this lot may work optimally at 0.1-0.5 μg/mL. Immunoprecipitation: 4 μg immunoprecipitates IRS-1 from 500 μg of 3T3/A31 RIPA lysate. Positive control: 3T3/A31 cell lysate. Load 20 μg of reduced lysate per lane for minigels. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
-
Target Species of Antibody: Rat
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, 0.05 % sodium azide, 40 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
-
- Target
- IRS1 (Insulin Receptor Substrate 1 (IRS1))
- Alternative Name
- IRS1 / IRS-1 (IRS1 Products)
- Synonyms
- HIRS-1 antibody, G972R antibody, IRS-1 antibody, IRS1IRM antibody, irs1 antibody, irsu antibody, IRS1 antibody, irs-1 antibody, irs1-a antibody, insulin receptor substrate 1 antibody, insulin receptor substrate 2 antibody, insulin receptor substrate 1 S homeolog antibody, IRS1 antibody, Irs1 antibody, irs2 antibody, irs1.S antibody
- Background
-
Name/Gene ID: IRS1
Synonyms: IRS1, IRS-1, HIRS-1, Insulin receptor substrate 1 - Gene ID
- 3667
- UniProt
- P35568
- Pathways
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
-