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Details for Product No. ABIN233754

NF-Y (N-Term) antibody

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Antigen
Epitope
»Alternatives N-Term
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Polyclonal
Application
»Alternatives Western Blotting (WB), Gel Shift (GS)
Pubmed 4 references available
Catalog no. ABIN233754
Quantity 100 µL
Price
361.90 $   Plus shipping costs $45.00 and if applicable $26.40 dry ice
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Immunogen NF-Y (A subunit specific) peptide corresponding to a region near the of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Characteristics Concentration Definition: by Refractometry
Purification Antiserum
Background Nuclear transcription factor Y subunit alpha is a protein that in humans is encoded by the NFYA gene. The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. Anti-NF-Y is ideal for investigators involved in transcription factor research.
Synonyms: Nuclear transcription factor Y subunit alpha Nuclear transcription factor Y subunit A NF-YA CAAT box DNA-binding protein subunit A
Gene ID 4800
NCBI Accession CAI20286.1
UniProt P23511
Research Area Transcription Factors
Application Notes Anti-NF-Y (A subunit) Antibody was assayed by immunoblot and found to be reactive against NF-Y (A subunit specific) at a dilution of 1:6,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] Goat).  Anti-NF-Y (A subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y.  Negligible reactivity was observed against the B subunit of NF-Y.   Anti-NF-Y (A subunit) Antibody was also tested in a gel supershift assay and found to be reactive against NF-Y using 1.0 to 2.0 µl per assay. Specific conditions should be optimized by user.
Restrictions For Research Use only
Format Liquid
Concentration 90 mg/mL
Preservative Sodium azide
Storage -20 °C
Klick, Hines: "Mechanisms regulating human FMO3 transcription." in: Drug metabolism reviews, Vol. 39, Issue 2-3, pp. 419-42, 2007 (PubMed).

Bennett, Seo, Datta et al.: "Selective binding of sterol regulatory element-binding protein isoforms and co-regulatory proteins to promoters for lipid metabolic genes in liver." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15628-37, 2008 (PubMed).

Gurtner, Fuschi, Magi et al.: "NF-Y dependent epigenetic modifications discriminate between proliferating and postmitotic tissue." in: PLoS ONE, Vol. 3, Issue 4, pp. e2047, 2008 (PubMed).

Manni, Caretti, Artuso et al.: "Posttranslational regulation of NF-YA modulates NF-Y transcriptional activity." in: Molecular biology of the cell, Vol. 19, Issue 12, pp. 5203-13, 2008 (PubMed).

Alternatives for antigen "NF-Y", type "Antibodies"
Hosts (7)
Reactivities (5)
Applications (6), (5), (1), (1), (1)
Epitopes (5), (1), (1)
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