NF-Y (N-Term) antibody

Details for Product No. ABIN233754
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Antigen
Epitope
N-Term
(5), (1), (1)
Reactivity
Human
(6)
Host
Rabbit
(7)
Clonality
Polyclonal
Application
Western Blotting (WB), Gel Shift (GS)
(6), (5), (1), (1), (1)
Pubmed 4 references available
Quantity 100 µL
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Catalog No. ABIN233754
361.90 $
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Immunogen NF-Y (A subunit specific) peptide corresponding to a region near the of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Characteristics Concentration Definition: by Refractometry
Purification Antiserum
Background Nuclear transcription factor Y subunit alpha is a protein that in humans is encoded by the NFYA gene. The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. Anti-NF-Y is ideal for investigators involved in transcription factor research.
Synonyms: Nuclear transcription factor Y subunit alpha Nuclear transcription factor Y subunit A NF-YA CAAT box DNA-binding protein subunit A
Gene ID 4800
UniProt P23511
Research Area Transcription Factors
Application Notes Anti-NF-Y (A subunit) Antibody was assayed by immunoblot and found to be reactive against NF-Y (A subunit specific) at a dilution of 1:6,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] Goat).  Anti-NF-Y (A subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y.  Negligible reactivity was observed against the B subunit of NF-Y.   Anti-NF-Y (A subunit) Antibody was also tested in a gel supershift assay and found to be reactive against NF-Y using 1.0 to 2.0 µl per assay. Specific conditions should be optimized by user.
Restrictions For Research Use only
Format Liquid
Concentration 90 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-NF-Y (N-Term) antibody CHO-7 cells were cultured in the absence (I) or presence (S) of cholesterol. Equivalent aliquots of chromatin from each sample containing ~50 ug of total protein were subjected to analysis by SDSPAGE and immunoblot. After transfer, the membrane was probed using anti-NF-YA as the primary antibody at a 1:1000 dilution, followed by reaction with HRP conjugated Goat-anti-Rabbit IgG [H&L]. Signal was developed using an ECL kit followed by autoradiography using a 30 exposure. The antibody recognizes NF-YA as a doublet of ~ 42 kDa in size.
anti-NF-Y (N-Term) antibody (2) anti-NF-Y (N-Term) antibody (Image 2)
General Klick, Hines: "Mechanisms regulating human FMO3 transcription." in: Drug metabolism reviews, Vol. 39, Issue 2-3, pp. 419-42, 2007 (PubMed).

Bennett, Seo, Datta et al.: "Selective binding of sterol regulatory element-binding protein isoforms and co-regulatory proteins to promoters for lipid metabolic genes in liver." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15628-37, 2008 (PubMed).

Gurtner, Fuschi, Magi et al.: "NF-Y dependent epigenetic modifications discriminate between proliferating and postmitotic tissue." in: PLoS ONE, Vol. 3, Issue 4, pp. e2047, 2008 (PubMed).

Manni, Caretti, Artuso et al.: "Posttranslational regulation of NF-YA modulates NF-Y transcriptional activity." in: Molecular biology of the cell, Vol. 19, Issue 12, pp. 5203-13, 2008 (PubMed).

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