NF-Y (N-Term) antibody

Antigen: NF-Y

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Gel Shift (GS)

Catalog no.: ABIN233754

Additional Information

Gene ID: 4800

UniProt: P23511

Immunogen: NF-Y (A subunit specific) peptide corresponding to a region near the of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Format: Liquid

Characteristics: Concentration Definition: by Refractometry

Application Details

Application Notes: This product was also tested in a gel supershift assay and found to be reactive against NF-Y using 1.0 to 2.0 µl per assay.
ELISA Dilution: 1:20,000 - 1:100,000 Gel Shift Dilution: Use 2.0 µL
Immunohistochemistry Dilution: 1:400
Western Blot Dilution: 1:2,000 - 1:10,000

Concentration: 90 mg/mL

Purification: Sterile filtered, Antiserum

Preservative: 0.01% (w/v) Sodium azide

Storage: Expiration date is one (1) year from date of opening. None. Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Research Area: Transcription Factors

Restrictions: For Research Use only

Images

CHO-7 cells were cultured in the absence (I) or presence (S) of cholesterol. Equivalent aliquots of chromatin from each sample containing ~50 ug of total protein were subjected to analysis by SDSPAGE and immunoblot. After transfer, the membrane was probed using anti-NF-YA as the primary antibody at a 1:1000 dilution, followed by reaction with HRP conjugated Goat-anti-Rabbit IgG [H&L]. Signal was developed using an ECL kit followed by autoradiography using a 30 exposure. The antibody recognizes NF-YA as a doublet of ~ 42 kDa in size.

anti-NF-Y (N-Term) antibody (Image 2)

Publications

Klick, Hines: "Mechanisms regulating human FMO3 transcription." in: Drug metabolism reviews, Vol. 39, Issue 2-3, pp. 419-42, 2007 (PubMed).

Bennett, Seo, Datta et al.: "Selective binding of sterol regulatory element-binding protein isoforms and co-regulatory proteins to promoters for lipid metabolic genes in liver." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15628-37, 2008 (PubMed).

Gurtner, Fuschi, Magi et al.: "NF-Y dependent epigenetic modifications discriminate between proliferating and postmitotic tissue." in: PLoS ONE, Vol. 3, Issue 4, pp. e2047, 2008 (PubMed).

Manni, Caretti, Artuso et al.: "Posttranslational regulation of NF-YA modulates NF-Y transcriptional activity." in: Molecular biology of the cell, Vol. 19, Issue 12, pp. 5203-13, 2008 (PubMed).