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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
NF-Y (N-Term) antibody
Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Gel Shift (GS)
|4 references available|
|Quantity||100 µL (90 mg/mL) (Variants)|
|Price||345.40 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||NF-Y (A subunit specific) peptide corresponding to a region near the of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).|
|Characteristics||Concentration Definition: by Refractometry|
This product was also tested in a gel supershift assay and found to be reactive against NF-Y using 1.0 to 2.0 µl per assay.
ELISA Dilution: 1:20,000 - 1:100,000 Gel Shift Dilution: Use 2.0 µL
Immunohistochemistry Dilution: 1:400
Western Blot Dilution: 1:2,000 - 1:10,000
|Purification||Sterile filtered, Antiserum|
|Preservative||0.01% (w/v) Sodium azide|
|Storage||Expiration date is one (1) year from date of opening. None. Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Research Area||Transcription Factors|
|Restrictions||For Research Use only|
Klick, Hines: "Mechanisms regulating human FMO3 transcription." in: Drug metabolism reviews, Vol. 39, Issue 2-3, pp. 419-42, 2007 (PubMed).
Bennett, Seo, Datta et al.: "Selective binding of sterol regulatory element-binding protein isoforms and co-regulatory proteins to promoters for lipid metabolic genes in liver." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15628-37, 2008 (PubMed).
Gurtner, Fuschi, Magi et al.: "NF-Y dependent epigenetic modifications discriminate between proliferating and postmitotic tissue." in: PLoS ONE, Vol. 3, Issue 4, pp. e2047, 2008 (PubMed).
Manni, Caretti, Artuso et al.: "Posttranslational regulation of NF-YA modulates NF-Y transcriptional activity." in: Molecular biology of the cell, Vol. 19, Issue 12, pp. 5203-13, 2008 (PubMed).
|Applications||ELISA (7), Western Blotting (WB) (6), Gel Shift (GS) (3), Immunoprecipitation (IP) (2), Immunoelectrophoresis (IEP) (1)|
|Epitopes||N-Term (3), Subunit A (1), Subunit B (1)|