You are viewing an incomplete version of our website. Please click to reload the website as full version.

NF-Y (N-Term) antibody

Details for Product No. ABIN233754, Supplier: Login to see New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Gel Shift (GS), Western Blotting (WB)
(3), (3), (1), (1)
Pubmed 4 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 100 μL
Shipping to United States ( )
Availability Will be delivered in 2 to 3 Business Days
Immunogen NF-Y (A subunit specific) peptide corresponding to a region near the of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Characteristics Concentration Definition: by Refractometry
Purification Antiserum
Background Nuclear transcription factor Y subunit alpha is a protein that in humans is encoded by the NFYA gene. The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. Anti-NF-Y is ideal for investigators involved in transcription factor research.
Synonyms: Nuclear transcription factor Y subunit alpha Nuclear transcription factor Y subunit A NF-YA CAAT box DNA-binding protein subunit A
Gene ID 4800
UniProt P23511
Research Area Transcription Factors
Application Notes Anti-NF-Y (A subunit) Antibody was assayed by immunoblot and found to be reactive against NF-Y (A subunit specific) at a dilution of 1:6,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] Goat).  Anti-NF-Y (A subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y.  Negligible reactivity was observed against the B subunit of NF-Y.   Anti-NF-Y (A subunit) Antibody was also tested in a gel supershift assay and found to be reactive against NF-Y using 1.0 to 2.0 µl per assay. Specific conditions should be optimized by user.
Restrictions For Research Use only
Format Liquid
Concentration 90 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Immunohistochemistry (IHC) image for anti-NF-Y (N-Term) antibody (ABIN233754) CHO-7 cells were cultured in the absence (I) or presence (S) of cholesterol. Equivale...
 image for anti-NF-Y (N-Term) antibody (ABIN233754) anti-NF-Y (N-Term) antibody (Image 2)
Product cited in: Manni, Caretti, Artuso et al.: "Posttranslational regulation of NF-YA modulates NF-Y transcriptional activity." in: Molecular biology of the cell, Vol. 19, Issue 12, pp. 5203-13, 2008 (PubMed).

Gurtner, Fuschi, Magi et al.: "NF-Y dependent epigenetic modifications discriminate between proliferating and postmitotic tissue." in: PLoS ONE, Vol. 3, Issue 4, pp. e2047, 2008 (PubMed).

Bennett, Seo, Datta et al.: "Selective binding of sterol regulatory element-binding protein isoforms and co-regulatory proteins to promoters for lipid metabolic genes in liver." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15628-37, 2008 (PubMed).

Background publications Klick, Hines: "Mechanisms regulating human FMO3 transcription." in: Drug metabolism reviews, Vol. 39, Issue 2-3, pp. 419-42, 2007 (PubMed).

Catalog No. ABIN233754
Plus shipping costs $45.00 and $21.00 dry ice

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)