F-Box Protein 4 (FBXO4) antibody

Antigen: F-Box Protein 4 (FBXO4)

Synonyms: FBX4, FLJ10141, DKFZp547N213, Fbx4, AI851261, AW494535, 1700096C12Rik, FBXO4, MGC127616, fbx4

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human, Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233769

Additional Information

Alternative name: F-box only protein 4 (FBX4)

Gene ID: NP_598860

Swiss-Prot: Q8CHQ0

Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide derived from sequences unique to the N-terminus of FBX4 and conserved between the human and murine proteins (see link below for the full-length sequence of the mouse gene product).

Format: Liquid (sterile filtered)

Description: Skp/Cullin/F-box (SCF) complexes are part of a large family of E3 ubiquitin ligases in which F-box proteins determine substrate specificity. Substrate selection by F-box proteins may also be aided by accessory cofactors. The F-box only protein 4, FBX4, interacts via its F-box with SKP1 and CUL1 to form SCF FBX4 ubiquitin ligase. The activity of the SCF FBX4 complex can be regulated by alpha B crystallin, a small heat shock protein which serves as a specificity cofactor for FBX4 dependent ubiquitination. FBX4 together with alpha B crystallin (SCF F B X 4 -alpha B c r y s t a l l i n ) promotes the ubiquitination of cyclin D1 phosphorylated at Thr-286. In addition, the SCF F B X 4 -alpha B c r y s t a l l i n ubiquitin ligase has been implicated in the ubiquitination of unknown substrates in desmin-related myopathy. SCF FBX4 , in the absence of alpha B crystallin, also ubiquitinates the telomeric protein PIN2/TRF1.

Specificity: This antiserum is directed against human and mouse FBX4. Reactivity against homologues from other sources is not known.

Application Details

Application Notes: This antiserum has been tested for use in western blotting and immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44 kDa in size by western blotting in the appropriate cell lysate or extract.

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.

Restrictions: For Research Use only

Images

Whole cell extracts prepared from U2OS cells, or insect Sf9 cells expressing Flag- FBX4, were resolved by SDSPAGE and transferred to nitrocellulose. FBX4 was detected using antibodies-online

Immunocytochemistry staining of NIH-3T3 cells with antibodies-online

Publications

Publications: Barbash, Zamfirova, Lin et al.: "Mutations in Fbx4 inhibit dimerization of the SCF(Fbx4) ligase and contribute to cyclin D1 overexpression in human cancer." in: Cancer cell, Vol. 14, Issue 1, pp. 68-78, 2008 (PubMed).

Pontano, Aggarwal, Barbash et al.: "Genotoxic stress-induced cyclin D1 phosphorylation and proteolysis are required for genomic stability." in: Molecular and cellular biology, Vol. 28, Issue 23, pp. 7245-58, 2008 (PubMed).

Barbash, Lee, Diehl: "Phosphorylation-dependent regulation of SCF(Fbx4) dimerization and activity involves a novel component, 14-3-3ɛ" in: Oncogene, 2011 (PubMed).