Cul3 (N-Term specific) antibody

Antigen: Cul3

Epitope: N-Term specific

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233772

Additional Information

Alternative name: Cul3 (N-terminal specific)

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a syntheticpeptide corresponding to amino acids 1-12 of Human Cul3 (N-terminus) coupled to KLH. Protein Sequence: Human Cullin 3 (768 aa 88.9 kDa) 1 msnlskgtgs rkdtkmrira fpmtmdekyv nsiwdllkna iqeiqrknns glsfeelyrn 61 aytmvlhkhg eklytglrev vtehlinkvr edvlnslnnn flqtlnqawn dhqtamvmir 121 dilmymdrvy vqqnnvenvy nlgliifrdq vvrygcirdh lrqtlldmia rerkgevvdr 181 gairnacqml milglegrsv yeedfeapfl emsaeffqme sqkflaensa svyikkvear 241 ineeiervmh cldksteepi vkvverelis khmktiveme nsglvhmlkn gktedlgcmy 301 klfsrvpngl ktmcecmssy lreqgkalvs eegegknpvd yiqglldlks rfdrfllesf 361 nndrlfkqti agdfeyflnl nsrspeylsl fiddklkkgv kglteqevet ildkamvlfr 421 fmqekdvfer yykqhlarrl ltnksvsdds eknmisklkt ecgcqftskl egmfrdmsis 481 nttmdefrqh lqatgvslgg vdltvrvltt gywptqsatp kcnippaprh afeifrrfyl 541 akhsgrqltl qhhmgsadln atfygpvkke dgsevgvgga qvtgsntrkh ilqvstfqmt 601 ilmlfnnrek ytfeeiqqet diperelvra lqslacgkpt qrvltkepks keienghift 661 vndqftsklh rvkiqtvaak qgesdperke trqkvdddrk heieaaivri mksrkkmqhn 721 vlvaevtqql karflpspvv ikkrieglie reylartped rkvytyva

Format: Liquid (sterile filtered)

Description: Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING proteinROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 3 is anessential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination ofproteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul3 serves as a rigidscaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul3 may also contribute to catalysis through positioningof the substrate and the ubiquitin-conjugating enzyme. Unlike Cul1 and Cul2, Cul3 seems not to be a part of the SCF complexconsisting of CUL1, RBX1, SKP1 and SKP2. Cul3 also interacts with RNF7 and is part of a complex with TIP120A/CAND1,Cyclin E and RBX1.

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterilefiltration. This product reacts with human Cullin 3. Cross reactivity is expected against mouse Cul3 based on sequencehomology. Cross reactivity with other human cullins is unlikely based on sequence homology.

Application Details

Application Notes: Recommended Dilutions: ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:500 - 1:1,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized. Note: This antibody reacts with human Cul3 by western blot and immunoprecipitation. The antibodyimmunoprecipitates in vitro translated product and protein fromcell lysates (using HeLa or NIH-3T3). An 88.9 kDa bandcorresponding to human Cul3 is detected. Most cell linesexpressing Cul3 can be used as a positive control.Researchers should determine optimal titers for otherapplications.

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: None.

Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extendedstorage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date isone (1) year from date of opening.

Restrictions: For Research Use only

Images

Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a

Publications

Publications: Shiraishi, Zhou, Aoki et al.: "TBP-interacting protein 120B (TIP120B)/cullin-associated and neddylation-dissociated 2 (CAND2) inhibits SCF-dependent ubiquitination of myogenin and accelerates myogenic differentiation." in: The Journal of biological chemistry, Vol. 282, Issue 12, pp. 9017-28, 2007 (PubMed).