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Cullin 4A (CUL4A) (N-Term), (AA 1-20) antibody

Details for Product No. ABIN233773
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Antigen
Synonyms zgc:64071, CUL4A, 2810470J21Rik, AW495282, RGD1563853
Epitope
N-Term, AA 1-20
(14), (7), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity
Human
(52), (23), (16), (12), (12), (12), (1)
Host
Rabbit
(48), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunoprecipitation (IP)
(37), (21), (10), (9), (9), (9), (8), (3), (2), (1), (1)
Pubmed 4 references available
Quantity 100 µL
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Catalog No. ABIN233773
361.90 $
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Immunogen This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1-20 of Human Cul4A coupled to KLH.
Characteristics Concentration Definition: by Refractometry
Purification Antiserum
Alternative Name Cul4A
Background Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates.  Cullin 4a is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul4A serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits.  Cul4A may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul4A also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1.
Synonyms: 2810470J21Rik antibody, AW495282 antibody, CUL 4A antibody, CUL4A antibody, Cul4a protein antibody, MGC36573 antibody, MGC64071 antibody
Gene ID 8451
NCBI Accession 38176422
UniProt Q13619
Application Notes This antibody reacts with human Cul4A by western blot and immunoprecipitation.  The antibody immunoprecipitates protein from cell lysates (using HeLa, U2OS and others).  This antibody also co-immunoprecipitates at least 8 subunits of COP9, DDB1 and CAND1.  An 87.6 kDa band corresponding to human Cul4A is detected.  Most cell lines expressing Cul4A can be used as a positive control.  Researchers should determine optimal titers for other applications.
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Cullin 4A (CUL4A) (N-Term), (AA 1-20) antibody Immunohistochemistry. anti-CUL4 antibody was diluted 1:500 to detect CUL4 in human testes tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
General Li, Jia, Kapur et al.: "Cul4A targets p27 for degradation and regulates proliferation, cell cycle exit, and differentiation during erythropoiesis." in: Blood, Vol. 107, Issue 11, pp. 4291-9, 2006 (PubMed).

Nishitani, Shiomi, Iida et al.: "CDK inhibitor p21 is degraded by a proliferating cell nuclear antigen-coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation." in: The Journal of biological chemistry, Vol. 283, Issue 43, pp. 29045-52, 2008 (PubMed).

Abbas, Sivaprasad, Terai et al.: "PCNA-dependent regulation of p21 ubiquitylation and degradation via the CRL4Cdt2 ubiquitin ligase complex." in: Genes & development, Vol. 22, Issue 18, pp. 2496-506, 2008 (PubMed).

Leung-Pineda, Huh, Piwnica-Worms: "DDB1 targets Chk1 to the Cul4 E3 ligase complex in normal cycling cells and in cells experiencing replication stress." in: Cancer research, Vol. 69, Issue 6, pp. 2630-7, 2009 (PubMed).

Validation Images
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