Cullin 4A (CUL4A) (N-Term) antibody

Antigen: Cullin 4A (CUL4A)

Synonyms: AW495282, MGC36573, 2810470J21Rik, RGD1563853, MGC64071, zgc:64071, CUL4A

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233773

Additional Information

Alternative name: Cul4A (N-terminal specific)

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1-20 of Human Cul4A (N-terminus) coupled to KLH.

Format: Liquid (sterile filtered)

Description: Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 4a is an essential componentof the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cellcycle progression, signal transduction and transcription. In the SCF complex, cul4A serves as a rigid scaffold that organizes theSKP1-F-box protein and RBX1 subunits. Cul4A may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul4A also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1.

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 4A. Cross reactivity is expected against mouse Cul4A based on a high degree ofsequence homology. Cross reactivity with other human cullins may occur.

Application Details

Application Notes: This antibody reacts with human Cul4A by western blot and immunoprecipitation. The antibodyimmunoprecipitates protein from cell lysates (using HeLa, U2OSand others). This antibody also co-immunoprecipitates at least 8subunits of COP9, DDB1 and CAND1. An 87.6 kDa bandcorresponding to human Cul4A is detected. Most cell linesexpressing Cul4A can be used as a positive control. Researchers should determine optimal titers for other applications. Dilutions: ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:500 - 1:1,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: None.

Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage.Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product isstable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date ofopening.

Restrictions: For Research Use only

Images

Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a

Publications

Publications: Li, Jia, Kapur et al.: "Cul4A targets p27 for degradation and regulates proliferation, cell cycle exit, and differentiation during erythropoiesis." in: Blood, Vol. 107, Issue 11, pp. 4291-9, 2006 (PubMed).

Nishitani, Shiomi, Iida et al.: "CDK inhibitor p21 is degraded by a proliferating cell nuclear antigen-coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation." in: The Journal of biological chemistry, Vol. 283, Issue 43, pp. 29045-52, 2008 (PubMed).

Abbas, Sivaprasad, Terai et al.: "PCNA-dependent regulation of p21 ubiquitylation and degradation via the CRL4Cdt2 ubiquitin ligase complex." in: Genes & development, Vol. 22, Issue 18, pp. 2496-506, 2008 (PubMed).

Leung-Pineda, Huh, Piwnica-Worms: "DDB1 targets Chk1 to the Cul4 E3 ligase complex in normal cycling cells and in cells experiencing replication stress." in: Cancer research, Vol. 69, Issue 6, pp. 2630-7, 2009 (PubMed).