Cullin 4A (CUL4A) (N-Term,AA 1-20) antibody

Antigen: Cullin 4A (CUL4A)

Synonyms: AW495282, MGC36573, 2810470J21Rik, RGD1563853, MGC64071, zgc:64071, CUL4A

Binding Site: N-Term,AA 1-20

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN233773

Additional Information

Alternative name: Cul4A

Gene ID: 8451

UniProt: Q13619

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1-20 of Human Cul4A coupled to KLH.

Format: Liquid

Description: Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 4a is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul4A serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul4A may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul4A also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1.

Characteristics: Concentration Definition: by Refractometry

Application Details

Application Notes: This antibody reacts with human Cul4A by western blot and immunoprecipitation. The antibody immunoprecipitates protein from cell lysates (using HeLa, U2OS and others). This antibody also co-immunoprecipitates at least 8 subunits of COP9, DDB1 and CAND1. An 87.6 kDa band corresponding to human Cul4A is detected. Most cell lines expressing Cul4A can be used as a positive control. Researchers should determine optimal titers for other applications.
ELISA Dilution: 1:2,000 - 1:10,000
Western Blot Dilution: 1:500 - 1:1,000

Concentration: 85 mg/mL

Purification: Sterile filtered, Antiserum

Preservative: 0.01% (w/v) Sodium azide

Storage: Expiration date is one (1) year from date of opening. None. Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Restrictions: For Research Use only

Images

Immunohistochemistry. anti-CUL4 antibody was diluted 1:500 to detect CUL4 in human testes tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Publications

Li, Jia, Kapur et al.: "Cul4A targets p27 for degradation and regulates proliferation, cell cycle exit, and differentiation during erythropoiesis." in: Blood, Vol. 107, Issue 11, pp. 4291-9, 2006 (PubMed).

Nishitani, Shiomi, Iida et al.: "CDK inhibitor p21 is degraded by a proliferating cell nuclear antigen-coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation." in: The Journal of biological chemistry, Vol. 283, Issue 43, pp. 29045-52, 2008 (PubMed).

Abbas, Sivaprasad, Terai et al.: "PCNA-dependent regulation of p21 ubiquitylation and degradation via the CRL4Cdt2 ubiquitin ligase complex." in: Genes & development, Vol. 22, Issue 18, pp. 2496-506, 2008 (PubMed).

Leung-Pineda, Huh, Piwnica-Worms: "DDB1 targets Chk1 to the Cul4 E3 ligase complex in normal cycling cells and in cells experiencing replication stress." in: Cancer research, Vol. 69, Issue 6, pp. 2630-7, 2009 (PubMed).