Cullin 7 (CUL7) (C-Term) antibody

Antigen: Cullin 7 (CUL7)

Synonyms: KIAA0076, dJ20C7.5, p185, p193, AA409809, 2510004L20Rik, C230011P08Rik, CUL7, Cullin-7, DKFZp469G0910

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233775

Additional Information

Alternative name: Cul7 (C-terminal specific)

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a syntheticpeptide corresponding to amino acids 1679-1698 of Human Cul7 (C-terminus) coupled to KLH.

Format: Liquid (sterile filtered)

Description: Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING proteinROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 7 is acomponent of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation oftarget proteins. Cullin 7 seems to be involved proteosomal degradation of proteins involved in endothelial proliferation and/ordifferentiation. Cullin 7 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1, FBXW8 and GLMN isoform 1. Cullin 7interacts with a complex of SKP1 and FBXW8, but not with SKP1 alone.

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterilefiltration. This product reacts with human and mouse Cullin 7. Cross reactivity with other human cullins may occur.

Application Details

Application Notes: This antibody reacts with human and mouse Cul7 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and also immunoprecipitates protein from cell lysates (using HeLa, NIH-3T3,and others). This antibody also co-immunoprecipitates associated proteins in overexpressed CUL7 systems. A 191.2 kDa band corresponding to human Cul7 is detected. Most cell lines expressing Cul7 can be used as a positive control. Researchers should determine optimal titers for other applications. Dilutions: ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:500 - 1:1,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: None.

Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extendedstorage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date isone (1) year from date of opening.

Restrictions: For Research Use only

Images

Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a