PARC (H7AP1) (C-Term specific) antibody

Antigen: PARC (H7AP1)

Epitope: C-Term specific

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233776

Additional Information

Alternative name: PARC (C-terminal specific)

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a syntheticpeptide corresponding to amino acids 2503-2517 of Human PARC (C-terminus) coupled to KLH.

Format: Liquid (sterile filtered)

Description: Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING proteinROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. PARC is a cullinfamily member that functions as a cytoplasmic anchor protein in p53-associated protein complexes. PARC regulates thesubcellular localization of p53 and subsequent function. PARC forms a complex with p53 in the cytoplasm of unstressed cellsand interacts with UBCH7 and UBCH8. PARC shows a cytoplasmic localization and is ubiquitously expressed in all tissueswith highest expression in testis brain and kidney.

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterilefiltration. This product reacts with human and mouse PARC. Cross reactivity with PARC from other sources is not known.

Application Details

Application Notes: This antibody reacts with human and mouse PARC by western blot and immunoprecipitation. The antibody immunoprecipitates protein from cell lysates (using HeLa,NIH-3T3, and others). To date co-immuno-precipitation using 35 S- IP has been negative. A 281.2 kDa band corresponding to humanPARC is detected. Most cell lines expressing PARC can be used as a positive control. Researchers should determine optimal titers for other applications. Dilutions: ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:500 - 1:1,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: None.

Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extendedstorage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date isone (1) year from date of opening.

Restrictions: For Research Use only

Images

Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a