p127-DDB1 (internal) antibody

Antigen: p127-DDB1

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233778

Additional Information

Alternative name: p127-DDB1 (internal)

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a syntheticpeptide corresponding to amino acids 198-213 of Human DDB1 (internal) coupled to KLH. Protein Sequence: Human DDB1 (1140 aa 127.0. kDa) 1 msynyvvtaq kptavngcvt ghftsaedln lliakntrle iyvvtaeglr pvkevgmygk 61 iavmelfrpk geskdllfil takynacile ykqsgesidi itrahgnvqd rigrpsetgi 121 igiidpecrm iglrlydglf kvipldrdnk elkafnirle elhvidvkfl ygcqapticf 181 vyqdpqgrhv ktyevsl rek efnkgpwkqe nve aeasmvi avpepfggai iigqesityh 241 ngdkylaiap piikqstivc hnrvdpngsr yllgdmegrl fmlllekeeq mdgtvtlkdl 301 rvellgetsi aecltyldng vvfvgsrlgd sqlvklnvds neqgsyvvam etftnlgpiv 361 dmcvvdlerq gqgqlvtcsg afkegslrii rngigiheha sidlpgikgl wplrsdpnre … 661 ssnhklvfsn vnlkevnymc plnsdgypds lalannstlt igtideiqkl hirtvplyes 721 prkicyqevs qcfgvlssri evqdtsggtt alrpsastqa lsssvssskl fssstaphet 781 sfgeevevhn lliidqhtfe vlhahqflqn eyalslvsck lgkdpntyfi vgtamvypee 841 aepkqgrivv fqysdgklqt vaekevkgav ysmvefngkl lasinstvrl yewttekdvr 901 tecnhynnim alylktkgdf ilvgdlmrsv lllaykpmeg nfeeiardfn pnwmsaveil 961 dddnflgaen afnlfvcqkd saattdeerq hlqevglfhl gefvnvfchg slvmqnlget 1021 stptqgsvlf gtvngmiglv tslseswynl lldmqnrlnk viksvgkieh sfwrsfhter 1081 ktepatgfid gdliesfldi srpkmqevva nlqyddgsgm kreataddli kvveeltrih

Format: Liquid (sterile filtered)

Description: DDB1 is also known as damage-specific DNA binding protein 1, DDB p127 subunit, DDBa, UV-damaged DNA-binding protein 1, UV-DDB 1, Xeroderma pigmentosum group E complementing protein, XPCe, X-associatedprotein 1 and XAP-1. The DDB1 gene encodes the large subunit (p127) of DNA damage-binding protein, which is aheterodimer, composed of a large and a small subunit (p48 DDB2). This nuclear protein functions in nucleotide-excision repairresulting from UV-damaged DNA by binding to pyrimidine dimers. Its defective activity causes the repair defect in the patientswith xeroderma pigmentosum complementation group E (XPE). XP-E is a rare human autosomal recessive diseasecharacterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases,neurological abnormalities. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is inthis gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the sameregion as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients.

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterilefiltration. This product reacts with human and mouse DDB1. Cross reactivity with DDB1 from other sources is not known.

Application Details

Application Notes: Recommended Dilutions: ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:500 - 1:1,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized. Note: This antibody reacts with human and mouse DDB1 by western blot and immunoprecipitation. Theantibody immunoprecipitates in vitro translated protein andprotein from cell lysates (using HeLa, NIH-3T3, and others).Coimmunoprecipitation of related proteins has not been tested.A 127.0 kDa band corresponding to human DDB1 is detected.Most cell lines expressing DDB1 can be used as a positivecontrol. Researchers should determine optimal titers for otherapplications.

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: None.

Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extendedstorage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date isone (1) year from date of opening.

Restrictions: For Research Use only

Images

Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a