P48-DDB2 (C-Term specific) antibody

Antigen: P48-DDB2

Epitope: C-Term specific

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233779

Additional Information

Alternative name: p48-DDB2 (C-terminal specific)

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a syntheticpeptide corresponding to amino acids 419-427 of Human DDB2 (C-terminal) coupled to KLH. Protein Sequence: Human DDB2 (427 aa 47.8 kDa) 1 mapkkrpetq ktseivlrpr nkrsrsplel epeakklcak gsgpsrrcds dclwvglagp 61 qilppcrsiv rtlhqhklgr aswpsvqqgl qqsflhtlds yrilqkaapf drratslawh 121 pthpstvavg skggdimlwn fgikdkptfi kgigaggsit glkfnplntn qfyassmegt 181 trlqdfkgni lrvfassdti niwfcsldvs assrmvvtgd nvgnvillnm dgkelwnlrm 241 hkkkvthval npccdwflat asvdqtvkiw dlrqvrgkas flyslphrhp vnaacfspdg 301 arllttdqks eirvysasqw dcplgliphp hrhfqhltpi kaawhprynl ivvgrypdpn 361 fksctpyelr tidvfdgnsg kmmcqlydpe ssgisslnef npmgdtlasa mgyhiliw sq 421 eeartrk

Format: Liquid (sterile filtered)

Description: DDB2 is also known as Damage-specific DNA binding protein 2, DDB p48 subunit, DDBb, UV-damaged DNA-binding protein 2 and UV-DDB 2. The DDB2 gene encodes the small subunit (p48) of DNA damage-binding protein, which is a heterodimer, composed of a large (p127 DDB1) and a small subunit. The DDB2 subunit appears to berequired for DNA binding. This nuclear protein functions in nucleotide-excision repair resulting from UV-damaged DNA bybinding to pyrimidine dimers. Its defective activity causes the repair defect in the patients with xeroderma pigmentosumcomplementation group E (XPE). XP-E is a rare human autosomal recessive disease characterized by solar sensitivity, highpredisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. However,it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding thelarge subunit.

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterilefiltration. This product reacts with human DDB2. Cross reactivity with DDB2 from other sources is not known.

Application Details

Application Notes: Recommended Dilutions: ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:500 - 1:1,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized. Note: This antibody reacts with human DDB2 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from celllysates (using 293T, and others). Coimmunoprecipitation of related proteins has not been tested. A 47.8 kDa band corresponding to human DDB2 is detected. Most cell lines expressing DDB2 can be used as a positive control. Researchers should determine optimaltiters for other applications.

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: None.

Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extendedstorage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date isone (1) year from date of opening.

Restrictions: For Research Use only

Images

anti-P48-DDB2 (C-Term specific) antibody

Publications

Publications: Abbas, Sivaprasad, Terai et al.: "PCNA-dependent regulation of p21 ubiquitylation and degradation via the CRL4Cdt2 ubiquitin ligase complex." in: Genes & development, Vol. 22, Issue 18, pp. 2496-506, 2008 (PubMed).