p48-DDB2 (AA 419-427,C-Term) antibody

Antigen: p48-DDB2

Binding Site: AA 419-427,C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN233779

Additional Information

Gene ID: 1643

UniProt: Q92466

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 419-427 of Human DDB2 coupled to KLH.

Format: Liquid

Description: DDB2 is also known as Damage-specific DNA binding protein 2, DDB p48 subunit, DDBb, UV-damaged DNA-binding protein 2 and UV-DDB 2. The DDB2 gene encodes the small subunit (p48) of DNA damage-binding protein, which is a heterodimer, composed of a large (p127 DDB1) and a small subunit. The DDB2 subunit appears to be required for DNA binding. This nuclear protein functions in nucleotide-excision repair resulting from UV-damaged DNA by binding to pyrimidine dimers. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). XP-E is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the large subunit.

Characteristics: Concentration Definition: by Refractometry

Application Details

Application Notes: This antibody reacts with human DDB2 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using 293T, and others). Coimmunoprecipitation of related proteins has not been tested. A 47.8 kDa band corresponding to human DDB2 is detected. Most cell lines expressing DDB2 can be used as a positive control. Researchers should determine optimal titers for other applications.
ELISA Dilution: 1:2,000 - 1:10,000
Western Blot Dilution: 1:500 - 1:1,000

Concentration: 85 mg/mL

Purification: Sterile filtered, Antiserum

Preservative: 0.01% (w/v) Sodium azide

Storage: Expiration date is one (1) year from date of opening. None. Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Restrictions: For Research Use only

Images

anti-p48-DDB2 (AA 419-427,C-Term) antibody

Publications

Abbas, Sivaprasad, Terai et al.: "PCNA-dependent regulation of p21 ubiquitylation and degradation via the CRL4Cdt2 ubiquitin ligase complex." in: Genes & development, Vol. 22, Issue 18, pp. 2496-506, 2008 (PubMed).