Cullin-Associated and Neddylation-Dissociated 2 (Putative) (CAND2) (C-Term specific) antibody

Antigen: Cullin-Associated and Neddylation-Dissociated 2 (Putative) (CAND2)

Synonyms: CAND2, Tp120b, TIP120B, KIAA0667

Epitope: C-Term specific

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233781

Additional Information

Alternative name: CAND2 (C-terminal specific)

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a syntheticpeptide corresponding to amino acids 1130-1143 of Human CAND2/TIP120B (C-terminal) coupled to KLH. Protein Sequence: Human CAND2 (TIP120B) (1143 aa 125.4 kDa) 1 mstaafhiss llekmtssdk dfrfmatsdl mselqkdsiq ldedserkvv kmllrlledk 61 ngevqnlavk wlgvplgafh asllhcllpq lssprlavrk ravgalghla aacstdlfve 121 ladhlldrlp gprvptspta irtliqclgs vgrqaghrlg ahldrlvplv edfcnlddde 181 lresclqafe aflrkcpkem gphvpnvtsl clqyikhdpn ynydsdedee qmetedsefs 241 eqesedeysd dddmswkvrr aaakciaali ssrpdllpdf hctlapvlir rfkereenvk … 901 vkflisdqph pidpllksfi gefmeslqdp dlnvrratla ffnsavhnkp slvrdllddi 961 lpllyqetki rrdlirevem gpfkhtvddg ldvrkaafec mysllesclg qldiceflnh 1021 vedglkdhyd irmltfimva rlatlcpapv lqrvdrliep lratctakvk agsvkqefek 1081 qdelkrsamr avaalltipe vgkspimadf ssqirsnpel aalfesiqk d sasapstdsm 1141 els

Format: Liquid (sterile filtered)

Description: CAND2 is also known as TIP120B, and TATA-binding protein-interacting protein 120B. While bothCAND1 (TIP120A) and CAND2 (TIP120B) are TATA-binding proteins and form complexes with various nuclear proteinsinvolved in the control of eukaryotic gene transcription, CAND2 (TIP120B) is expressed specifically in muscle and heart tissue.This is contrary to the ubiquitous expression of CAND1 (TIP120A). TIP120 homologs exist in various higher eukaryotesincluding D. melanogaster, C. elegans, and A. thaliana. TIP120B is 60% identical in amino acid sequence to TIP120A.

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterilefiltration. This product reacts with human, rat and mouse CAND2/TIP120B. Cross reactivity does occur with human, rat andmouse CAND1/TIP120A. Cross reactivity with CAND2 from other sources is not known.

Application Details

Application Notes: Recommended Dilutions: ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:500 - 1:1,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized. Note: This antibody reacts with human, rat and mouse CAND2 by western blot and immunoprecipita-tion. Theantibody immunoprecipitates in vitro translated protein and proteinfrom transfected cell lysates (using HeLa and NIH-3T3, andothers). Coimmunoprecipitation of related proteins has not been tested. A 125.4 kDa band corresponding to human CAND2 isdetected. CAND2 is specifically expressed in muscle and hearttissue. Researchers should determine optimal titers for otherapplications.

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: None.

Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extendedstorage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date isone (1) year from date of opening.

Restrictions: For Research Use only

Images

Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a