ROC2 (C-Term specific) antibody

Antigen: ROC2

Epitope: C-Term specific

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233783

Additional Information

Alternative name: ROC2 (C-terminal specific)

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a syntheticpeptide corresponding to amino acids 102-113 of Human ROC2 (C-terminal) coupled to KLH. Protein Sequence: Human ROC2 (113 aa 12.6 kDa) 1 madvedgeet calashsgss gsksggdkmf slkkwnavam wswdvecdtc aicrvqvmda 61 clrcqaenkq edcvvvwgec nhsfhnccms lwvkqnnrcp l cqqdwvvqr igk

Format: Liquid (sterile filtered)

Description: ROC1 also known as RING-box protein 2, Rbx2, RING finger protein 7, Regulator of cullins 2, CKII beta- binding protein 1, and CKBBP1, is a probable component of the SCF (SKP1-CUL1-F-box protein) and the CBC(VHL) (CUL2-elongingBC-VHL) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteinsinvolved in cell cycle progression, signal transduction and transcription. ROC2 appears to recruit the E2 ubiquitination enzymethrough the RING-type zinc finger in a manner similar to CDC34, and brings it into close proximity to the substrate. ROC2 may play arole in protecting cells from apoptosis induced by redox agents. ROC2 has a cytoplasmic and nuclear localization and is expressed inheart, liver, skeletal muscle and pancreas tissues, and at very low levels in brain, placenta and lung. 1,10-phenanthroline inducesROC2 expression. The RING-type zinc finger domain is essential for ubiquitin ligase activity. Phosphorylation by CK2 is required forefficient degradation of NFKBIA and CDKN1B.

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human, mouse, C.elgans and zebra fish ROC2. Cross reactivity may also occur with ROC2 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins suchas ROC1 and APC11.

Application Details

Application Notes: Recommended Dilutions: ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:500 - 1:1,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized. Note: This antibody reacts with human ROC2 by western blot and immunoprecipitation. The antibodyimmunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others).Coimmunoprecipitation of related proteins does occur. A 12.6 kDa band corresponding to human ROC2 is detected. Most cell lines expressing ROC2 can be used as a positive control. Researchersshould determine optimal titers for other applications.

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: None.

Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage.Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product isstable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date ofopening.

Restrictions: For Research Use only

Images

Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a