Anaphase Promoting Complex Subunit 11 (ANAPC11) (C-Term) antibody

Antigen: Anaphase Promoting Complex Subunit 11 (ANAPC11)

Synonyms: APC11, Apc11p, MGC882, HSPC214, anapc11, RGD1561880, ANAPC11, F10A16.17, F10A16_17, R75218, 1110011I19Rik, MGC127621, zgc:153671, si:dkey-237i9.4, DKFZp459G2130

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233784

Additional Information

Alternative name: APC11(C-terminal specific)

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a syntheticpeptide corresponding to amino acids 76-84 of Human APC11 (C-terminal) coupled to KLH. Protein Sequence: Human APC11 (84 aa 9.8 kDa) 1 mkvkikcwng vatwlwvand encgicrmaf ngccpdckvp gddcplvwgq cshcfhmhci 61 lkwlhaqqvq qhcpm crqew kfke

Format: Liquid (sterile filtered)

Description: APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11,Hepatocellular carcinoma associated RING finger protein, and HSPC214. APC11 is a component of the anaphase promotingcomplex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G 1 phase of the cell cycle. APC11 may function to recruit the E2 ubiquitin-conjugating enzymes to the complex. APC11 interacts with the cullin domain of ANAPC2 and also interacts with UBE2D2. APC11 shows both a cytoplasmic and nuclear localization.APC11 is expressed at high levels in skeletal muscle and heart, in moderate levels in brain, kidney, and liver, and at low levelsin colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte. APC11 is a member of the RING-typezinc finger family and is auto-ubiquitinylated.

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterilefiltration. This product reacts with human and mouse APC11. Cross reactivity may also occur with APC11 from other sources.Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1and ROC2.

Application Details

Application Notes: Recommended Dilutions: ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:500 - 1:1,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized. Note: This antibody reacts with human APC11 by western blot and immunoprecipitation. The antibodyimmunoprecipitates in vitro translated protein and protein fromoverexpressing cell lysates (using HeLa and NIH-3T3, and others).Coimmunoprecipitation of related proteins (APC2) does occur. A9.8 kDa band corresponding to human APC11 is detected. Most celllines or tissues expressing APC11 can be used as a positive control.Researchers should determine optimal titers for other applications.

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: None.

Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extendedstorage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date isone (1) year from date of opening.

Restrictions: For Research Use only

Images

Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a