APC2 (C-Term specific) antibody

Antigen: APC2

Epitope: C-Term specific

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233785

Additional Information

Alternative name: APC2 (C-terminal specific)

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a syntheticpeptide corresponding to amino acids 810-822 of Human APC2 (C-terminal) coupled to KLH. Protein Sequence: Human APC2 (822 aa 93.8 kDa) 1 maaavvvaeg dsdsrpgqel lvawntvstg lvppaalglv ssrtsgavpp keeelraave 61 vlrghglhsv leewfvevlq ndlqanispe fwnaisqcen sadepqclll lldafglles 121 rldpylrsle llekwtrlgl lmgtgaqglr eevhtmlrgv lffstprtfq emiqrlygcf 181 lrvymqskrk geggtdpele geldsryarr ryyrllqspl cagcssdkqq cwcrqaleqf 241 hqlsqvlhrl sllervsaea vtttlhqvtr ermedrcrge yersflrefh kwiervvgwl 301 gkvflqdgpa rpaspeagnt lrrwrchvqr ffyriyaslr ieelfsivrd fpdsrpaied 361 lkyclertdq rqqllvslka aletrllhpg vntcdiitly isaikalrvl dpsmvileva 421 cepirrylrt redtvrqiva gltgdsdgtg dlavelsktd pasletgqds eddsgepedw 481 vpdpvdadpg kssskrrssd iisllvsiyg skdlfineyr slladrllhq fsfspereir 541 nvellklrfg eapmhfcevm lkdmadsrri nanireedek rpaeeqppfg vyavilssef 601 wppfkdekle vpediraale ayckkyeqlk amrtlswkht lglvtmdvel adrtlsvavt 661 pvqavillyf qdqaswtlee lskavkmpva llrrrmsvwl qqgvlreepp gtfsvieeer 721 pqdrdnmvli dsddesdsgm asqadqkeee lllfwtyiqa mltnleslsl driynmlrmf 781 vvtgpalaei dlqelqgylq kkvrdqqlv y sagvyrlpkn cs

Format: Liquid (sterile filtered)

Description: APC2, also known as Anaphase promoting complex subunit 2, APC2, Cyclosome subunit 2, andANAPC2, is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligasethat controls progression through mitosis and the G 1 phase of the cell cycle. The APC/cyclosome protein complex promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitors, whichare subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains at leasteleven subunits. The composition of APC is highly conserved in organisms from yeast to humans. APC2 is a cullin familymember that interacts through the cullin domain with ANAPC11 and UBCH10.

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterilefiltration. This product reacts with human APC2. Cross reactivity may also occur with APC2 from other sources. Sufficientsequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 andROC2.

Application Details

Application Notes: Recommended Dilutions: ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:500 - 1:1,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized. Note: This antibody reacts with human APC2 by western blot and immunoprecipitation. The antibodyimmunoprecipitates in vitro translated protein and protein fromoverexpressing cell lysates (using HeLa and NIH-3T3, and others).Coimmunoprecipitation of related proteins (APC11) does occur. A93.8 kDa band corresponding to human APC2 is detected. Most celllines or tissues expressing APC2 can be used as a positive control.Researchers should determine optimal titers for other applications.

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: None.

Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extendedstorage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date isone (1) year from date of opening.

Restrictions: For Research Use only

Images

Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a