Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (C-Term specific) antibody

Antigen: Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20)

Synonyms: CDC20A, p55CDC, MGC102824, bA276H19.3, C87100, 2310042N09Rik, X-FZY, cdc20a, fizzy1, p55cdc, MGC75919, ba276h19.3

Epitope: C-Term specific

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233786

Additional Information

Alternative name: CDC20 (fizzy) (C-terminal specific)

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a syntheticpeptide corresponding to amino acids 486-499 of Human CDC20 (fizzy) (C-terminal) coupled to KLH. Protein Sequence: Human CDC20 (fizzy) (499 aa 54.7 kDa) 1 maqfafesdl hsllqldapi pnapparwqr kakeaagpap spmraanrsh sagrtpgrtp 61 gkssskvqtt pskpggdryi phrsaaqmev asfllskenq sensqtptkk ehqkawalnl 21 ngfdveeaki lrlsgkpqna pegyqnrlkv lysqkatpgs srktcryips lpdrildape 181 irndyylnlv dwssgnvlav aldnsvylws assgdilqll qmeqpgeyis svawikegny 241 lavgtssaev qlwdvqqqkr lrnmtshsar vgslswnsyi lssgsrsghi hhhdvrvaeh 301 hvatlsghsq evcglrwapd grhlasggnd nlvnvwpsap geggwvplqt ftqhqgavka 361 vawcpwqsnv latgggtsdr hiriwnvcsg aclsavdahs qvcsilwsph ykelisghgf 421 aqnqlviwky ptmakvaelk ghtsrvlslt mspdgatvas aaadetlrlw rcfeldparr 481 rerek asaak sslihqgir

Format: Liquid (sterile filtered)

Description: CDC20, also known as fizzy, Cell division cycle protein 20 homolog, and p55CDC is required forfull ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity uponthe complex. CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cellcycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosomeseparation. CDC20 is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The phosphorylated form of CDC20 interactswith APC/C. Synthesis is initiated at G 1 /S, protein level peaks in M phase and protein is abruptly degraded at M/G 1 transition.

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterilefiltration. This product reacts with human CDC20 (fizzy). Cross reactivity may also occur with CDC20 from other sources.

Application Details

Application Notes: Recommended Dilutions: ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:500 - 1:1,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized. Note: This antibody reacts with human CDC20 (fizzy) by western blot and immunoprecipitation. The antibodyimmunoprecipitates in vitro translated protein and protein fromoverexpressing cell lysates (using HeLa and NIH-3T3, and others).Coimmunoprecipitation of related proteins has not been determined.A 54.7 kDa band corresponding to human CDC20 (fizzy) isdetected. Most cell lines or tissues expressing CDC20 can be usedas a positive control. Researchers should determine optimal titersfor other applications.

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: None.

Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extendedstorage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date isone (1) year from date of opening.

Restrictions: For Research Use only

Images

Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a

Publications

Publications: Yamamuro, Kano, Naito: "Functions of FZR1 and CDC20, activators of the anaphase-promoting complex, during meiotic maturation of swine oocytes." in: Biology of reproduction, Vol. 79, Issue 6, pp. 1202-9, 2008 (PubMed).