C-Krox antibody

Antigen: C-Krox

Synonyms: Thpok, Zfp67, c-Krox, MGC102577

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human, Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233787

Additional Information

Alternative name: cKrox

Swiss-Prot: Q64321

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of mouse cKrox protein.

Format: Liquid (sterile filtered)

Description: CKrox (also known as Zfp67 or ThPOK) functions as a transcription regulator that acts as a key modulator of lineage commitment for immature T-cell precursors. cKrox is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. cKrox is a known transcriptional repressor of the collagen COL1A1 and COL1A2 genes and may also function as a repressor of fibronectin and possibly other extracellular matrix genes. cKrox is located within the nucleus and is expressed in multiple tissues and organs including skin, thymus and peripheral T-cells.

Specificity: This antibody is directed against mouse cKrox. The product is delipidated and defibrinated antiserum. A BLAST analysis was used to suggest cross-reactivity with cKrox from mouse, human and rat based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Application Details

Application Notes: This antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80 kDa in size corresponding to cKrox by western blotting in the appropriate cell lysate or extract. Lysates from mouse thymocytes are recommended for use as positive controls.

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at 4° C prior to opening. Reconstitute with 0.1 ml of deionized water. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening product.

Restrictions: For Research Use only

Images

Western blot using antibodies-online's anti-cKrox antibody to detect endogenous cKrox in mouse thymocyte whole cell lysates. The left lane is a control with no primary antibody. The center lane is a reagent control. The right lane shows a band at 80 kDa corresponding to mouse cKrox protein. The membrane was probed with the primary antibody at a 1:500 dilution. Personal Communication, L. Wang and R. Bossulet, National Cancer Institute, Bethesda, MD.