Selenophosphate Synthetase 1 (SEPHS1) (SPS1) antibody

Antigen: Selenophosphate Synthetase 1 (SEPHS1)

Synonyms: SPS, SELD, SPS1, MGC4980, AA589574, AI505014, AW111620, 1110046B24Rik, MGC68922, sephs1, SEPHS1, zgc:55304, wu:fc49b09, MGC69442, MGC142727, Sps1, DKFZp469M1021

Epitope: SPS1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human, Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233788

Additional Information

Alternative name: Selenophosphate Synthetase 1 (SPS1)

Gene ID: BAC32163

Swiss-Prot: Q8BH69

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant protein corresponding to mouse SPS1.

Format: Liquid (sterile filtered)

Description: Selenophosphate synthetase 1 (SPS1) (also known as selinide water dikinase or selenium donor protein 1) is the homolog of the SelD gene product in E. coli. In E. coli, SelD generates the selenium donor for selenocysteine tRNA biosynthesis. The function of SPS1 in mammals is still unknown but appears to differ from that of E. coli SelD.

Specificity: This antibody is directed against mouse SPS1. The product is delipidated and defibrinated antiserum. A BLAST analysis was used to suggest cross-reactivity with SPS1 from mouse, human and rat based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Application Details

Application Notes: This antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to SPS1 by western blotting in the appropriate cell lysate or extract. Dilutions: ELISA 15,000 - 120,000 WESTERN BLOT 1500 - 12,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at 4° C prior to opening. Reconstitute with 0.1 ml of deionized water. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening product.

Restrictions: For Research Use only

Images

Western blot using antibodies-online's anti- SPS1 antibody shows detection of endogenous SPS1 in NIH3T3 cell. No signal is seen in lysates from cells after pretreatment with SPS1 siRNA. Endogenous SPS1 can be detected in mouse kidney and liver tissue lysates. Negligible crossreactivity is seen against recombinant SPS2. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.