Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody

Antigen: Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A)

Synonyms: HIF1, MOP1, PASD8, bHLHe78, HIF-1alpha, HIF1-ALPHA, HIF1A, HIF-1a, HIF1a, hif-1a, hif1a

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human, Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233790

Additional Information

Alternative name: Hif-1a hydroxyP564

Gene ID: NP_001521

Swiss-Prot: Q16665

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region surrounding the P564 of human HIF-1 alpha .

Format: Liquid (sterile filtered)

Description: Tumor hypoxia often directly correlates with aggressive phenotype, metastasis progression and resistance to chemotherapy. HIF-1 transcription factors are dramatically induced in hypoxic areas and regulate the expression of genes necessary for tumor adaptation to conditions of low oxygen. The stabilization of HIF-1 alpha by hypoxia is critically dependent upon the hydroxylation of certain Proline residues that exist in the oxygen-dependent degradation domain of HIF-1 alpha . HIF factors are now considered an important therapeutic target for cancer intervention.

Specificity: This antibody is directed against human HIF-1 alpha HYDROXY P564 and is specific for the hydroxylated form of the protein. Minimal reactivity occurs with the non-hydroxylated form of the protein. A BLAST analysis was used to suggest cross-reactivity with HIF-1 alpha from human, monkey, mouse, rat, dog, bovine and Xenopus sources based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Application Details

Application Notes: This antibody has been tested for use in ELISA and western blotting. This antibody is specific for HIF-1 alpha hydroxylated at P564. Minimal cross-reactivity occurs with non-hydroxylated HIF-1 alpha. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 110 kDa in size corresponding to HIF-1 alpha HYDROXY P564 by western blotting in the appropriate cell lysate or extract.

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at 4° C prior to opening. Reconstitute with 0.1 ml of deionized water. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening product.

Restrictions: For Research Use only

Images

Western blot using antibodies-online's anti-HIF-1alpha HYDROXYP564 antibody shows detection (left panel) of hydroxylated HIF1-alpha in nuclear extracts of A549 cells treated with MG132 (a proteosome inhibitor). Hydroxyproline is not recognized on HIF1-alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF1-alpha. The asterisk marks a band approximately 110 kDa in size corresponding to HIF1-alpha. The primary antibody was used at a 1:1,000 dilution in 2% BLOTTO. Personal Communication, L. Neckers and O. Aprelikova, NCI, Bethesda, MD.