SARS-CoV Nonstructural Protein 8 (Nsp8) antibody

Antigen: SARS-CoV Nonstructural Protein 8 (Nsp8)

Clonality: Polyclonal

Host: Rabbit

Application: Neutralization (Neut), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233792

Additional Information

Gene ID: NP_828849, NP_828866

Immunogen: This whole rabbit serum was produced by repeated immunizations with a purified His- taggedrecombinant protein corresponding to full-length SARS-Coronavirus nsp8.

Format: Liquid (sterile filtered)

Description: The nonstructural protein 8 (nsp8)is one of the SARS-Coronavirus replicase cleaving products,encoded by ORF1a. Nsp8 is thought to be part of the viralreplication complex, which is associated with intracellularmembranes. No specific information on the function of nsp8is available.

Specificity: This antibody is directed against SARS-Coronavirus nsp8 protein. The product is neatantiserum. Cross reactivity with homologues from other sources has not been determined.

Application Details

Application Notes: This antibody has been tested for use in immunofluorescence microscopy, immunoelectronmicroscopy, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the enduser. Expect a band of approximately 22 kDa in size corresponding to SARS-CoV nsp8 by western blotting in theappropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, wereinfected with SARS-CoV-Fr1 strain for 1 h at 37

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: None.

Storage: Store vial at -20° C prior to opening.Dilute only prior to immediate use. For extended storagealiquot contents and freeze at -20° C or below. Avoid cyclesof freezing and thawing. Expiration date is one (1) year fromdate of opening.

Restrictions: For Research Use only

Images

Immunoprecipitation followed by western blotting using antibodies-online Immunochemicals Anti-nsp8 shows a predominant band at 21.8 kDa corresponding to full length SARS protein (panel A). Immunofluorescence Microscopy using anti-nsp8 6-h post infection of Vero-E6 cells (Panel B). For detection Cy3 conjugated Goat-anti- Rabbit IgG MX (611-104-122) was used. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.