SARS-CoV Nonstructural Protein 13 (Nsp13) antibody

Antigen: SARS-CoV Nonstructural Protein 13 (Nsp13)

Clonality: Polyclonal

Host: Rabbit

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233793

Additional Information

Gene ID: NP_828849, NP_828870

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a BSA-coupled synthetic peptide corresponding to the C-terminus (amino acid residues 584-601) of the SARS Coronavirusnonstructural protein 13.

Format: Liquid (sterile filtered)

Description: The coronavirus nonstructuralprotein 13 (nsp13) is one of the SARS-Coronavirus replicasecleaving products encoded by ORF1b. Nsp13 is thought to bepart of the viral replication complex, which is associated withintracellular membranes. Nsp13 contains a C-terminalNTPase/Helicase domain and an N-terminal putative zinc-binding motif.

Specificity: This antibody is directed against SARS-Coronavirus nsp13 protein. The product is neatantiserum. Cross reactivity with homologues from other sources has not been determined.

Application Details

Application Notes: This antibody has been tested for use in western blotting, immunofluorescence microscopy andimmunoelectron microscopy. Specific conditions for reactivity should be optimized by the end user. Expect a band ofapproximately 67 kDa in size corresponding to SARS-CoV nsp13 by western blotting in the appropriate cell lysate orextract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1strain for 1 h at 37

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: None.

Storage: Store vial at -20° C prior to opening.Dilute only prior to immediate use. For extended storagealiquot contents and freeze at -20° C or below. Avoid cyclesof freezing and thawing. Expiration date is one (1) year fromdate of opening.

Restrictions: For Research Use only

Images

Immunofluorescence Microscopy using antibodies-online Immunochemicals anti-SARS-CoV nsp13 antibody, 6-h post infection Vero-E6 cells. For detection Cy3 conjugated Goat-anti-Rabbit IgG MX (611-104-122) was used. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.