SARS-CoV Membrane (M) Protein antibody

Antigen: SARS-CoV Membrane (M) Protein

Clonality: Polyclonal

Host: Rabbit

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233794

Additional Information

Gene ID: NP_828849

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a BSA-coupled synthetic peptide corresponding to the C-terminus (amino acid residues 204-221) of the SARS Coronavirus Membrane protein.

Format: Liquid (sterile filtered)

Description: The coronavirus membraneprotein is a major structural component of the viral envelopeand a determinant of virus assembly.

Specificity: This antibody is directed against SARS-Coronavirus membrane protein. The product is neatantiserum. Cross reactivity with homologues from other sources has not been determined.

Application Details

Application Notes: This antibody has been tested for use in immunofluorescence microscopy, immunoprecipitation,immunoelectron microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 25 kDa in size corresponding to SARS-CoV membrane protein by western blotting inthe appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, wereinfected with SARS-CoV-Fr1 strain for 1 h at 37

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: None.

Storage: Store vial at -20° C prior to opening.Dilute only prior to immediate use. For extended storagealiquot contents and freeze at -20° C or below. Avoid cyclesof freezing and thawing. Expiration date is one (1) year fromdate of opening.

Restrictions: For Research Use only

Images

Immunofluorescence Microscopy using antibodies-online Immunochemicals anti- SARS-CoV Membrane protein antibody, 6-h post infection Vero-E6 cells. For detection Cy3 conjugated Goat-anti-Rabbit IgG MX (611-104-122) was used. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.