NFKB p65 (C-Term) antibody

Antigen: NFKB p65

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Gel Shift (GS)

Catalog no.: ABIN233796

Additional Information

Gene ID: 5970

UniProt: Q04206

Immunogen: NFkB p65 (Rel A) peptide corresponding to a region near the of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Format: Liquid

Description: Anti NFkB p65 Antibody recognizes NFKB p65 which is a component of NFKB. NFKB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkappaB bound to IkappaB. NFkappaB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkappaB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkappaBalpha. IkappaBalpha-binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.

Characteristics: Concentration Definition: by Refractometry

Application Details

Application Notes: Anti-NFkB p65 (Rel A) is suitable for the detection by immunoblot of human and mouse NFkB p65 (Rel A). This product was also tested in a gel supershift assay and found to be reactive against all p65 (Rel A) containing human, mouse or rat NFkB complexes using 0.5 to 1.0 µl per assay.
ELISA Dilution: 1:5,000 Gel Shift Dilution: 05 µL - 1.0 µL
Immunohistochemistry Dilution: 1:400
Western Blot Dilution: 1:2,000 - 1:5,000

Concentration: 80 mg/mL

Purification: Sterile filtered, Antiserum

Preservative: 0.01% (w/v) Sodium azide

Storage: Expiration date is one (1) year from date of opening. None. Store NF-kappaB antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Restrictions: For Research Use only

Images

anti-NFKB p65 (C-Term) antibody

anti-NFKB p65 (C-Term) antibody (Image 2)

anti-NFKB p65 (C-Term) antibody (Image 3)

Publications

Miller, Cheshire, Raab-Traub: "Interaction of tumor necrosis factor receptor-associated factor signaling proteins with the latent membrane protein 1 PXQXT motif is essential for induction of epidermal growth factor receptor expression." in: Molecular and cellular biology, Vol. 18, Issue 5, pp. 2835-44, 1998 (PubMed).

Feng, Cheng, Hsia et al.: "NF-kappaB inducible genes BCL-X and cyclin E promote immature B-cell proliferation and survival." in: Cellular immunology, Vol. 232, Issue 1-2, pp. 9-20, 2005 (PubMed).

Lou, Kaplowitz: "Glutathione depletion down-regulates tumor necrosis factor alpha-induced NF-kappaB activity via IkappaB kinase-dependent and -independent mechanisms." in: The Journal of biological chemistry, Vol. 282, Issue 40, pp. 29470-81, 2007 (PubMed).

Begley, Kasina, Mehra et al.: "CXCL5 promotes prostate cancer progression." in: Neoplasia (New York, N.Y.), Vol. 10, Issue 3, pp. 244-54, 2008 (PubMed).

Yoon, Korade, Carter: "Protein kinase A-induced phosphorylation of the p65 subunit of nuclear factor-kappaB promotes Schwann cell differentiation into a myelinating phenotype." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 14, pp. 3738-46, 2008 (PubMed).