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p65 antibody (Nuclear Factor-KB P65) (C-Term)

Details for Product anti-NFkBP65 Antibody No. ABIN233796, Supplier: Login to see
Antigen
  • NFKB3
  • p65
  • NFkB
Alternatives
anti-Human p65 antibody for Western Blotting
Epitope
C-Term
105
80
70
53
50
47
44
42
42
39
34
26
22
16
15
15
14
13
13
10
10
10
9
8
8
7
6
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1049
473
390
44
37
26
12
10
6
4
4
3
2
2
2
1
1
1
1
1
Host
Rabbit
974
81
13
8
2
Clonality
Polyclonal
Conjugate
This p65 antibody is un-conjugated
31
30
22
19
16
16
11
9
9
8
8
8
8
8
8
1
1
1
1
1
Application
Gel Shift (GS), Western Blotting (WB)
769
408
336
172
150
134
90
42
30
24
21
19
10
9
7
7
7
4
2
2
1
1
Supplier
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Immunogen NFkB p65 (Rel A) peptide corresponding to a region near the of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics Concentration Definition: by Refractometry
Purification Antiserum
Alternative Name NFKB p65 (NFkBP65 Antibody Abstract)
Background Anti NFkB p65 Antibody recognizes NFKB p65 which is a component of NFKB. NFKB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkappaB bound to IkappaB. NFkappaB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkappaB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkappaBalpha. IkappaBalpha-binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.
Synonyms: NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB, Nuclear factor NF-kappa-B p65 subunit
Gene ID 5970, 223468676
UniProt Q04206
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Anti-NFkB p65 (Rel A) is suitable for the detection by immunoblot of human and mouse NFkB p65 (Rel A).  This product was also tested in a gel supershift assay and found to be reactive against all p65 (Rel A) containing human, mouse or rat NFkB complexes using 0.5 to 1.0 µl per assay. 
Restrictions For Research Use only
Format Liquid
Concentration 80 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Immunohistochemistry (IHC) image for anti-p65 antibody (Nuclear Factor-KB P65) (C-Term) (ABIN233796) anti-Nuclear Factor-KB P65 (NFkBP65) (C-Term) antibody
 image for anti-p65 antibody (Nuclear Factor-KB P65) (C-Term) (ABIN233796) anti-Nuclear Factor-KB P65 (NFkBP65) (C-Term) antibody (Image 2)
 image for anti-p65 antibody (Nuclear Factor-KB P65) (C-Term) (ABIN233796) anti-Nuclear Factor-KB P65 (NFkBP65) (C-Term) antibody (Image 3)
Product cited in: Yoon, Korade, Carter: "Protein kinase A-induced phosphorylation of the p65 subunit of nuclear factor-kappaB promotes Schwann cell differentiation into a myelinating phenotype." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 14, pp. 3738-46, 2008 (PubMed).

Begley, Kasina, Mehra et al.: "CXCL5 promotes prostate cancer progression." in: Neoplasia (New York, N.Y.), Vol. 10, Issue 3, pp. 244-54, 2008 (PubMed).

Lou, Kaplowitz: "Glutathione depletion down-regulates tumor necrosis factor alpha-induced NF-kappaB activity via IkappaB kinase-dependent and -independent mechanisms." in: The Journal of biological chemistry, Vol. 282, Issue 40, pp. 29470-81, 2007 (PubMed).

Background publications Feng, Cheng, Hsia et al.: "NF-kappaB inducible genes BCL-X and cyclin E promote immature B-cell proliferation and survival." in: Cellular immunology, Vol. 232, Issue 1-2, pp. 9-20, 2005 (PubMed).

Miller, Cheshire, Raab-Traub: "Interaction of tumor necrosis factor receptor-associated factor signaling proteins with the latent membrane protein 1 PXQXT motif is essential for induction of epidermal growth factor receptor expression." in: Molecular and cellular biology, Vol. 18, Issue 5, pp. 2835-44, 1998 (PubMed).

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