Baculoviral IAP Repeat-Containing 5 (BIRC5) antibody

Antigen: Baculoviral IAP Repeat-Containing 5 (BIRC5)

Synonyms: API4, EPR-1, AP14, BIRC5, MGC133855, DKFZp468N162, Api4, TIAP, AAC-11, survivin40, bir5a, survivin, bir1-A, svv1, XBIR1, Xsvv1, birc5.1-A, xSurvivin, XSurvivin1, LOC398389, LOC398418, Su2, birc5.1-B, BIR1, Survivin, Survivin1, xSurvivin1, SIX, Su1, api4, svv2, birc5, epr-1, xSurvivin2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human, Rat (Rattus)

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233803

Additional Information

Alternative name: Survivin

Gene ID: NP_001159

Swiss-Prot: O15392

Immunogen: This IgG fraction antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human Survivin protein.

Format: Liquid (sterile filtered)

Description: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli, including members of the TNF family, and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel IAP protein was recently identified and designated Survivin (also referred to as apoptosis inhibitor 4 (API4) and TIAP). Survivin interacts with the processed form of caspase-3 and inhibits its proteolytic activity. Survivin is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas.

Specificity: This IgG fraction antibody is directed against human Survivin protein. The product was purified from monospecific antiserum by selective precipitation followed by ion exchange chromatography. Reactivity against Survivin from other sources has not been determined.

Synonyms: API4, EPR-1, AP14, BIRC5, MGC133855, DKFZp468N162, Api4, TIAP, AAC-11, survivin40, bir5a, survivin, bir1-A, svv1, XBIR1, Xsvv1, birc5.1-A, xSurvivin, XSurvivin1, LOC398389, LOC398418, Su2, birc5.1-B, BIR1, Survivin, Survivin1, xSurvivin1, SIX, Su1, api4, svv2, birc5, epr-1, xSurvivin2

Application Details

Application Notes: Recommended Dilutions: ELISA 1:10,000 - 1:40,000 WESTERN BLOT 1:200 - 1:2,000 IF MICROSCOPY 1:100 - 1:1,000 OTHER APPLICATIONS User Optimized. Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Paraformalde-hyde fixation is recommended for immunohistochemical staining. Expect a band at ~17 kDa in size corresponding to Survivin by western blotting in the appropriate cell lysate or extract.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.02% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Research Area: Cancer, Apoptosis/Necrosis, Glia marker, Neurology

Restrictions: For Research Use only

Images

Western blot using antibodies-online's IgG Fraction of anti-Survivin antibody shows detection of a single band at ~17 kDa corresponding to Survivin in MOLT4 cell lysates in the absence (lane A) and presence (lane B) of blocking peptide. Primary antibody was used at 1 ug/ml.

Immunohistochemical staining using antibodies-online's IgG Fraction of anti-Survivin antibody at 10 ug/ml shows detection of Survivin in MOLT4 cells. Cells were fixed in 4% paraformaldehyde.