Toll-Like Receptor 7 (TLR7) antibody

Antigen: Toll-Like Receptor 7 (TLR7)

Synonyms: RGD1563357, TLR7A

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN233806

Additional Information

Alternative name: TLR7

Gene ID: NP_573474

Swiss-Prot: P58681

Immunogen: This IgG fraction antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse toll-like receptor 7 (TLR7) protein.

Format: Liquid (sterile filtered)

Description: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. TLR7, like TLRs 3, 8, and 9, is localized in intracellular acidic compartments such as the phagolysosome and will recognize some single-stranded RNA viruses such as vesicular stomatitis virus (VSV) and influenza virus. Activation of TLR7 by VSV results in stimulation of the immune response including IFN secretion, suggesting the importance of TLR7 in virus recognition.

Specificity: This IgG fraction antibody is directed against human TLR7 protein. This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody reacts with TLR7 from human and mouse sources. Cross reactivity with TLR7 from other sources has not been determined.

Application Details

Application Notes: This IgG fraction antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~121 kDa in size corresponding to TLR7 by western blotting in the appropriate cell lysate or extract.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.02% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20o C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20o C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Research Area: Immunology, Innate Immunity, Transcription Factors, Signaling, Inflammation

Restrictions: For Research Use only

Images

Western blot using IgG fraction of anti-TLR7 antibody shows detection of a predominant band at ~121 kDa corresponding to TLR7 (arrowhead) in a Daudi whole cell lysate. The predicted MW of TLR7 is 121 kDa. TLR7 was detected using 0.5 ug/ml (lane A), 1.0 ug/ml (lane B) and 2 ug/ml (lane C) concentrations of primary antibody.