Toll-Like Receptor 10 (TLR10) antibody

Antigen: Toll-Like Receptor 10 (TLR10)

Synonyms: CD290, MGC104967, MGC126398, MGC126399, TLR10, MGC140623

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN233807

Additional Information

Alternative name: TLR10

Gene ID: NP_112218

Swiss-Prot: Q32MI7

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human toll-like receptor 10 protein.

Format: Liquid (sterile filtered)

Description: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. TLR10 is highly homologous to TLRs 1 and 6 and is most highly expressed in lymphoid tissues. Most recently genetic variation in TLR10 has been associated with contributing to asthma risk. It should be noted that TLR10 exists in mice only as a pseudogene.

Specificity: This IgG fraction antibody is directed against human TLR10 protein. This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is reactive with TLR10 from human sources. Cross reactivity with TLR10 from other sources has not been determined.

Application Details

Application Notes: This IgG fraction antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~75 kDa in size corresponding to TLR10 by western blotting in the appropriate cell lysate or extract.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.02% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20o C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20o C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Research Area: Immunology, Innate Immunity

Restrictions: For Research Use only

Images

Western blot using IgG fraction of anti-TLR10 antibody shows detection of a predominant band at ~75 kDa corresponding to TLR10 (arrowhead) inhuman lymph node whole cell lysate. The predicted MW of TLR10 is 95 kDa. TLR10 was detected using 0.5 ug/ml (lane A) and 1.0 ug/ml (lane B) concentrations of primary antibody.