Toll-Like Receptor 11 (Tlr11) antibody

Antigen: Toll-Like Receptor 11 (Tlr11)

Synonyms: Gm287, MGC129352, MGC129353, RGD1564460

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN233808

Additional Information

Alternative name: TLR11

Gene ID: NP_991388

Swiss-Prot: Q6R5P0

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of mouse toll-like receptor 11 (TLR11) protein.

Format: Liquid (sterile filtered)

Description: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. TLR11 is one of three mouse TLRs that lack a human ortholog. It is activated specifically by uropathogenic bacteria, and mice lacking TLR11 showed a much greater susceptability to uropathogenic infections, indicating a potentially important role for TLR11 in preventing infections in the urogenital system.

Specificity: This IgG fraction antibody is directed against human TLR11 protein. This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is reactive with TLR11 from human sources. Cross reactivity with TLR11 from other sources has not been determined.

Application Details

Application Notes: This IgG fraction antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~90 kDa in size corresponding to TLR11 by western blotting in the appropriate cell lysate or extract.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.02% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20o C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20o C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Research Area: Immunology, Innate Immunity, Signaling, Inflammation

Restrictions: For Research Use only

Images

Western blot using IgG fraction of anti-TLR11 antibody shows detection of a predominant band at ~90 kDa corresponding to TLR11 (arrowhead) in a RAW264.7 whole cell lysate. The predicted MW of TLR11 is 106 kDa. TLR11 was detected using 0.5 ug/ml (lane A) and 1.0 ug/ml (lane B) concentrations of primary antibody.