Toll-Like Receptor 1 (TLR1) antibody

Antigen: Toll-Like Receptor 1 (TLR1)

Synonyms: TIL, CD281, rsc786, KIAA0012, MGC104956, MGC126311, MGC126312, DKFZp547I0610, DKFZp564I0682

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN233810

Additional Information

Alternative name: TLR1

Gene ID: NP_003254

Swiss-Prot: Q15399

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human toll-like receptor 1 (TLR1) protein.

Format: Liquid (sterile filtered)

Description: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR1 is co-expressed with TLR2 on myeloid cells of the innate immune systems in lymphoid tissue such as monocytes and dendritic cells where they form heterodimers that can recognize triacylated lipoproteins.

Specificity: This IgG fraction antibody is directed against human TLR1 protein. This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is reactive with TLR1 from human and mouse sources. Cross reactivity with TLR1 from other sources has not been determined.

Application Details

Application Notes: This IgG fraction antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~90 kDa in size corresponding to TLR1 by western blotting in the appropriate cell lysate or extract.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.02% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20o C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20o C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Research Area: Immunology, Innate Immunity, Signaling, Inflammation

Restrictions: For Research Use only

Images

Western blot using IgG fraction of anti-TLR1 antibody shows detection of a predominant band at ~90 kDa corresponding to TLR1 (arrowhead) in mouse spleen tissue whole cell lysate. The predicted MW of TLR1 is 90 kDa. TLR1 was detected using 1.0 ug/ml (lane A, 2.0 ug/ml (lane B) and 4.0 ug/ml (lane C) concentrations of primary antibody.