HSP90 antibody (Lys294)

Catalog No. ABIN233817

Product Details anti-HSP90 Antibody

Target: HSP90 (Heat Shock Protein 90 (HSP90))

Binding Specificity: Lys294

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP90 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding K294 of human Hsp90.

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.  This antibody reacts strongly with the acetylated form of the immunizing peptide and shows minimal reactivity with the non-acetylated form when tested by ELISA.  To date, western blotting shows equivalent staining of lysates either treated or untreated with Trichostatin A (an HDAC inhibitor).  Therefore, western blotting results are not definitive for demonstrating the specificity of this reagent.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 90 kDa in size corresponding to Hsp90 protein by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-HSP90 antibody (ABIN233817)

Manca, Frisbie, LaGrange, Casey, Riethoven, Petrosyan: "The Role of Alcohol-Induced Golgi Fragmentation for Androgen Receptor Signaling in Prostate Cancer." in: Molecular cancer research : MCR, Vol. 17, Issue 1, pp. 225-237, (2019) (PubMed).

Deskin, Lasky, Zhuang, Shan: "Requirement of HDAC6 for activation of Notch1 by TGF-β1." in: Scientific reports, Vol. 6, pp. 31086, (2018) (PubMed).

Mo, Zhao, Liu, Hu, Chen, Pham, Wei, Liu, Zhou, Burgess, Pfaff, Caskey, Wu, Bai, Yang: "Aberrant GlyRS-HDAC6 interaction linked to axonal transport deficits in Charcot-Marie-Tooth neuropathy." in: Nature communications, Vol. 9, Issue 1, pp. 1007, (2018) (PubMed).

Dedes, Dedes, Imesch, von Bueren, Fink, Fedier: "Acquired vorinostat resistance shows partial cross-resistance to 'second-generation' HDAC inhibitors and correlates with loss of histone acetylation and apoptosis but not with altered HDAC and HAT activities." in: Anti-cancer drugs, Vol. 20, Issue 5, pp. 321-33, (2009) (PubMed).

Target Details for HSP90

Target: HSP90 (Heat Shock Protein 90 (HSP90))

Alternative Name: HSP 90 (HSP90 Products)

Synonyms: EL52 antibody, HSP86 antibody, HSP89A antibody, HSP90A antibody, HSP90N antibody, HSPC1 antibody, HSPCA antibody, HSPCAL1 antibody, HSPCAL4 antibody, HSPN antibody, Hsp89 antibody, Hsp90 antibody, LAP2 antibody, git10 antibody, swo1 antibody, HSP90 antibody, htpG antibody, SCBAC25F8.08 antibody, 23.m06066 antibody, 17.m07646 antibody, HSP90-1 antibody, 143198_at antibody, 83 antibody, 83K HSP antibody, DMHSP82 antibody, E(sev)3A antibody, E(sina)2 antibody, HSP82 antibody, HSP83 antibody, ORF1 antibody, Su(Raf)3A antibody, anon-EST:Liang-2.53 antibody, anon-WO0068693 antibody, anon-WO0140519.209 antibody, clone 2.53 antibody, en(lz)3C/4C antibody, hsp84 antibody, l(3)j5C2 antibody, ms(3)08445 antibody, stc antibody, DmelCG1242 antibody, CG1242 antibody, Hsp86 antibody, Hspca antibody, 86kDa antibody, 89kDa antibody, AL024080 antibody, AL024147 antibody, Hsp86-1 antibody, hsp4 antibody, hsp86 antibody, hsp89 antibody, hsp90 antibody, hsp90a antibody, hspc1 antibody, hspca antibody, hspn antibody, lap2 antibody, Hsp90alpha antibody, heat shock protein 90 alpha family class A member 1 antibody, heat shock protein Hsp90 antibody, Hsp90 chaperone antibody, Heat shock protein 90 antibody, heat shock protein 90 antibody, chaperone protein HtpG antibody, molecular chaperone HtpG antibody, Heat Shock Protein 90 antibody, uncharacterized LOC100384473 antibody, Heat shock protein 83 antibody, heat shock protein 90, alpha (cytosolic), class A member 1 antibody, heat shock protein 90 alpha family class B member 1 antibody, heat shock protein 90kDa alpha family class A member 1 L homeolog antibody, heat shock protein HSP 90-alpha antibody, HSP90AA1 antibody, hsp90 antibody, HSP90 antibody, daf-21 antibody, htpG antibody, SCO7516 antibody, MAP_RS10510 antibody, TP04_0646 antibody, TP01_0934 antibody, APH_RS03525 antibody, GbCGDNIH1_0315 antibody, MAV_2118 antibody, HSP90C antibody, BBOV_IV008400 antibody, BBOV_III007380 antibody, ACICU_00312 antibody, ECL_01244 antibody, YE105_C1172 antibody, pco153543(105) antibody, Hsp83 antibody, Hsp90aa1 antibody, HSP90AB1 antibody, hsp90aa1.1.L antibody, LOC108698781 antibody

Background: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Hsp90 is a member of the heat shock protein 90 family, the members of which are highly conserved between isoforms and species. Hsp90 functions as a molecular chaperone and has ATPase activity.  Hsp90 is a cytoplasmic protein that forms a homodimer in vivo and interacts with TOM34, AHSA1, HDAC6 and SMYD3.  Several signal transduction pathways depend on Hsp90 function, including pathways involving erbB2, hypoxia sensitivity (Hif1 alpha), and steroid hormone receptors (for example, androgen, progesterone, glucocorticoid, and aryl-hydrocarbon).  Recent reports show that Hsp90 from tumor cells has increased sensitivity to small molecule inhibitors (for example, 17AAG).  The mechanism of the differential sensitivity of Hsp90 from normal versus tumor cells is unknown, although mutation has been ruled out.  One possible mechanism may be differences in post-translational modification of tumor Hsp90.  K294 was found to be acetylated in purified Hsp90 from SkBr3 cells, a breast cancer cell line.
Synonyms: Heat shock protein HSP 90-alpha Gene names Name: HSP90AA1 Synonyms: HSP90A, HSPCA

Gene ID: 3320, 123678

UniProt: P07900

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals