RFP antibody (Red Fluorescent Protein)

Details for Product anti-RFP Antibody No. ABIN233863, Supplier: Log in to see
Antigen
Reactivity
Discosoma
82
50
36
10
7
2
1
1
Host
Rabbit
96
38
14
9
1
Clonality
Polyclonal
Conjugate
This RFP antibody is un-conjugated
9
7
6
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Western Blotting (WB)
133
72
61
53
33
29
7
5
4
3
2
1
1
1
1
1
1
Options
Supplier
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Immunogen Recombinant RFP expressed from E. coli
Isotype IgG
Purification Affinity purified
Alternative Name RFP (RFP Antibody Abstract)
Background Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. RFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or RFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP or RFP can fuse with proteins of interest without interfering significantly with their assembly and function.
Synonyms: Red Fluorescent Protein
Gene ID 3229046
UniProt P00273
Research Area Tags/Labels
Application Notes Western blotting (0.5-4 μg/mL). Based on researchers' feedback, it can also be used in immunoprecipitation (10-20 μg/mL) and immunofluorescence. However, the optimal conditions should be determined individually.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) affinity purified rabbit anti-RFP polyclonall antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 5 mM EDTA and 0.03 % proclin
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Red Fluorescent Protein (RFP) antibody (ABIN233863) Western blot analysis with recombinant RFP. Lane 1: 10 ng Lane 2: 50 ng Lane 3: 250 n...
Product cited in: Manzo, Silies, Gohl, Scott: "Motor neurons controlling fluid ingestion in Drosophila." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 16, pp. 6307-12, 2012 (PubMed).

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