Apolipoprotein E (APOE) antibody

Antigen: Apolipoprotein E (APOE)

Synonyms: AD2, LPG, LDLCQ5, MGC1571, AI255918, APOEA, APOE, ad2, apoprotein, MGC110064, im:7036787, wu:fb69a05, zgc:110064, apoe

Clonality: Monoclonal (E6D7)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN234327

Additional Information

Alternative name: Apolipoprotein E (Apo E)

Immunogen: Synthetic peptide spanning the polymorphic aa position 158 of Apo E

Format: Purified, Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: E6D7

Description: MAb to Apolipoprotein E Monoclonal Antibody to Apolipoprotein E (Apo E) Ascites

Specificity: Specific to Human Apo E. Does not cross-react with mouse and rabbit. Reacts to the E2, E3 and E4 isoforms of apolipoprotein E.

Application Details

Application Notes: Western blot (1:1,000?1:10,000) ELISA Immunoprecipitation Immunohistochemistry: (1:1,000?1:10,000) Frozen and formalin-fixed paraffin-embedded tissues. The staining intensity of formalin-fixed paraffin embedded tissues may be significantly improved by the pretreatment of: Formic acid (70%) for 10?30 minutes at room temperature. HIER (Heat Induced Epitope Retrieval) with buffers such as citrate. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: 1mg/ml (OD280nm)

Purification: Protein A chromatography. Source: Ascites

Buffer: PBS.

Storage: Short-term store at 2-8°C. Long term, aliquot and store at -20°C. Avoid multiple freeze/thaw cycles.

Research Area: Metabolism

Restrictions: For Research Use only

Publications

Publications: Munoz, Feldman: "Causes of Alzheimer's disease." in: CMAJ : Canadian Medical Association journal = journal de l'Association medicale canadienne, Vol. 162, Issue 1, pp. 65-72, 2001 (PubMed).