Apolipoprotein E (APOE) antibody
| Antigen |
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| Synonyms |
AD2, LPG, LDLCQ5, MGC1571, AI255918, APOEA, APOE, ad2, apoprotein, MGC110064, im:7036787, wu:fb69a05, zgc:110064, apoe |
| Clonality |
Monoclonal (E6D7) |
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Host
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Reactivity
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Conjugate
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Biotin (9), HRP (3), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Application
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 Alternatives ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
Western Blotting (WB) (101), ELISA (86), Immunohistochemistry (IHC) (28), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (25), Immunofluorescence (IF) (15), Immunoprecipitation (IP) (10), Flow Cytometry (FACS) (7), Immunodiffusion (ID) (7), Immunoelectrophoresis (IEP) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Radioimmunoassay (RIA) (5), Enzyme Immunoassay (EIA) (4), Immunocytochemistry (ICC) (4), Immunohistochemistry (Fixed) (IHC (fx)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Radial Immunodiffusion (RID) (2), Dot Blot (Dot) (1), Immunoassay (IA) (1), Immunoelectron Microscopy (IEM) (1), Ouchterlony Double Diffusion (ODD) (1), Thin Layer Immunoassay (TIA) (1), To be determined by user (TBD) (1)
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| Catalog no. |
ABIN234327 |
| Quantity |
100ul (1mg/ml (OD280nm)) |
| Price |
471.53 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Will be delivered in 4 to 5 Business Days |
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Alternative name
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Apolipoprotein E (Apo E)
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Immunogen
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Synthetic peptide spanning the polymorphic aa position 158 of Apo E
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Format
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Liquid
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Isotype
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IgG1
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Clone
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E6D7
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Description
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MAb to Apolipoprotein E Monoclonal Antibody to Apolipoprotein E (Apo E) Ascites
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Specificity
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Specific to Human Apo E. Does not cross-react with mouse and rabbit. Reacts to the E2, E3 and E4 isoforms of apolipoprotein E.
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Application Notes
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Western blot (1:1,000?1:10,000) ELISA Immunoprecipitation Immunohistochemistry: (1:1,000?1:10,000) Frozen and formalin-fixed paraffin-embedded tissues. The staining intensity of formalin-fixed paraffin embedded tissues may be significantly improved by the pretreatment of: Formic acid (70%) for 10?30 minutes at room temperature. HIER (Heat Induced Epitope Retrieval) with buffers such as citrate. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
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Concentration
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1mg/ml (OD280nm)
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Purification
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Source: Tissue culture supernatant
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Buffer
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PBS.
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Storage
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Short-term store at 2-8°C. Long term, aliquot and store at -20°C. Avoid multiple freeze/thaw cycles.
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Research Area
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Metabolism
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Restrictions
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For Research Use only
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Munoz, Feldman: "Causes of Alzheimer's disease." in: CMAJ : Canadian Medical Association journal = journal de l'Association medicale canadienne, Vol. 162, Issue 1, pp. 65-72, 2001 (PubMed).
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Alternatives for antigen "Apolipoprotein E (APOE)", type "Antibodies"
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Hosts
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Rabbit (73), Goat (54), Mouse (26)
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Reactivities
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Human (107), Mouse (Murine) (28), Rat (Rattus) (24), Rabbit (7), Hamster (4), Chimpanzee (1)
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Applications
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Western Blotting (WB) (101), ELISA (86), Immunohistochemistry (IHC) (28), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (25), Immunofluorescence (IF) (15), Immunoprecipitation (IP) (10), Flow Cytometry (FACS) (7), Immunodiffusion (ID) (7), Immunoelectrophoresis (IEP) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Radioimmunoassay (RIA) (5), Enzyme Immunoassay (EIA) (4), Immunocytochemistry (ICC) (4), Immunohistochemistry (Fixed) (IHC (fx)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Radial Immunodiffusion (RID) (2), Dot Blot (Dot) (1), Immunoassay (IA) (1), Immunoelectron Microscopy (IEM) (1), Ouchterlony Double Diffusion (ODD) (1), Thin Layer Immunoassay (TIA) (1), To be determined by user (TBD) (1)
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Conjugates
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Biotin (9), HRP (3), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Epitopes
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C-Term (24), N-Term (5), Isoform E3 (4), Internal Region (3), AA 140-160 (1)
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