Apolipoprotein E (APOE) antibody
| Antigen |
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| Synonyms |
AD2, LPG, LDLCQ5, MGC1571, AI255918, APOEA, APOE, ad2, apoprotein, MGC110064, im:7036787, wu:fb69a05, zgc:110064, apoe |
| Clonality |
Monoclonal (D6E10) |
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Host
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Reactivity
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Conjugate
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Biotin (7), HRP (3), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Application
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 Alternatives ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
Western Blotting (WB) (88), ELISA (77), Immunohistochemistry (IHC) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (24), Immunofluorescence (IF) (15), Immunoprecipitation (IP) (10), Immunodiffusion (ID) (7), Immunoelectrophoresis (IEP) (6), Flow Cytometry (FACS) (5), Radioimmunoassay (RIA) (5), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (Fixed) (IHC (fx)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Radial Immunodiffusion (RID) (2), Dot Blot (Dot) (1), Immunoassay (IA) (1), Immunoelectron Microscopy (IEM) (1), Ouchterlony Double Diffusion (ODD) (1), Thin Layer Immunoassay (TIA) (1), To be determined by user (TBD) (1)
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| Catalog no. |
ABIN234328 |
| Quantity |
0.2ml (1mg/ml) |
| Price |
860.20 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Will be delivered in 4 to 5 Business Days |
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Alternative name
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Apolipoprotein E (Apo E)
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Immunogen
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Synthetic peptide spanning the polymorphic aa position 158 of Apo E
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Format
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Liquid
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Isotype
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IgG1
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Clone
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D6E10
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Description
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MAb to Apolipoprotein E Monoclonal Antibody to Apolipoprotein E (Apo E) Tissue culture supernatant
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Specificity
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Reacts to the E2, E3 and E4 isoforms of Apolipoprotein E.
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Application Notes
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ELISA Western blot Immunoprecipitation Immunohistochemistry: Frozen and formalin-fixed paraffin-embedded tissues. The staining intensity of formalin-fixed paraffin embedded tissues may be significantly improved by the pretreatment of: Formic acid (70%) for 10-30 minutes at room temperature. HIER (Heat Induced Epitope Retrieval) with buffers. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
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Concentration
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1mg/ml
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Purification
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HPLC purified. Source: Ascites
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Buffer
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PBS. Preservative: 0.1% Sodium azide
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Storage
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Store at -20°C to -70°C. Aliquot to avoid multiple freeze/thaw cycles.
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Research Area
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Metabolism
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Restrictions
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For Research Use only
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Zunarelli, Nicoll, Migaldi et al.: "Apolipoprotein E polymorphism and breast carcinoma: correlation with cell proliferation indices and clinical outcome." in: Breast cancer research and treatment, Vol. 63, Issue 3, pp. 193-8, 2001 (PubMed).
Munoz, Feldman: "Causes of Alzheimer's disease." in: CMAJ : Canadian Medical Association journal = journal de l'Association medicale canadienne, Vol. 162, Issue 1, pp. 65-72, 2001 (PubMed).
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Alternatives for antigen "Apolipoprotein E (APOE)", type "Antibodies"
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Hosts
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Rabbit (62), Goat (55), Mouse (23)
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Reactivities
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Human (95), Mouse (Murine) (24), Rat (Rattus) (20), Rabbit (5), Hamster (4), Chimpanzee (1)
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Applications
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Western Blotting (WB) (88), ELISA (77), Immunohistochemistry (IHC) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (24), Immunofluorescence (IF) (15), Immunoprecipitation (IP) (10), Immunodiffusion (ID) (7), Immunoelectrophoresis (IEP) (6), Flow Cytometry (FACS) (5), Radioimmunoassay (RIA) (5), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (Fixed) (IHC (fx)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Radial Immunodiffusion (RID) (2), Dot Blot (Dot) (1), Immunoassay (IA) (1), Immunoelectron Microscopy (IEM) (1), Ouchterlony Double Diffusion (ODD) (1), Thin Layer Immunoassay (TIA) (1), To be determined by user (TBD) (1)
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Conjugates
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Biotin (7), HRP (3), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Epitopes
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C-Term (6), N-Term (5), AA 140-160 (1)
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2013 BioMed Central Research Awards
