B-Cell CLL/lymphoma 3 (BCL3) antibody

Antigen: B-Cell CLL/lymphoma 3 (BCL3)

Synonyms: BCL4, D19S37, Bcl-3, AI528691, BCL9-2, DLNB11, BCL3

Clonality: Monoclonal (B325H)

Host: Hamster

Reactivity: Human, Mouse (Murine)

Application: Western Blotting (WB)

Catalog no.: ABIN234363

Additional Information

Alternative name: Bcl-3

Immunogen: Recombinant mouse Bcl-3-6His

Format: Purified, Liquid

Isotype: IgG  (Matching secondary antibodies)

Clone: B325H

Description: MAb to Bcl-3 Monoclonal Antibody to Bcl-3

Specificity: Recognizes human and mouse Bcl-3. The gene encoding Bcl-3 was first identified as the gene altered in some cases of chronic B cell lymphocytic leukemia and was later found to belong to the I-kappa-B subfamily of inhibitors of NF-kappa-B transcription factors. Bcl-3 does not have DNA-binding activity, but it interacts with NF-kappa-B1 and NF-kappa-B2 which, when bound to DNA, repress gene transcription. Therefore, Bcl-3 may promote gene expression that is repressed by members of the NF-kappa-B family. Bcl-3 RNA is highly expressed in spleen and liver with lower levels of expression in heart, skeletal muscle, lung, kidney and testes.

Application Details

Application Notes: Suitable for use in Western blot (use as 2ug/ml) and ELISA. In Western blot, a band of approximately 46-52 kDa is detected. Positive control: Jurkat T cell lysate. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: Lot specific (OD280nm)

Purification: Protein G chromatography. Source: Cell culture

Buffer: PBS, pH 7.4.

Storage: Upon receipt, store at -20°C. Avoid multiple freeze/thaw cycles.

Research Area: Cancer

Restrictions: For Research Use only

Publications

Publications: Nolan, Fujita, Bhatia et al.: "The bcl-3 proto-oncogene encodes a nuclear I kappa B-like molecule that preferentially interacts with NF-kappa B p50 and p52 in a phosphorylation-dependent manner." in: Molecular and cellular biology, Vol. 13, Issue 6, pp. 3557-66, 1993 (PubMed).

Watanabe, Iwamura, Shinoda et al.: "Regulation of NFKB1 proteins by the candidate oncoprotein BCL-3: generation of NF-kappaB homodimers from the cytoplasmic pool of p50-p105 and nuclear translocation." in: The EMBO journal, Vol. 16, Issue 12, pp. 3609-20, 1997 (PubMed).

Mitchell, Hildeman, Kedl et al.: "Immunological adjuvants promote activated T cell survival via induction of Bcl-3." in: Nature immunology, Vol. 2, Issue 5, pp. 397-402, 2001 (PubMed).